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Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes

Saidman, SL and Duquesnoy, RJ and Zeevi, A and Fung, JJ and Starzl, TE and Demetris, AJ (1991) Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes. Hepatology, 13 (2). 239 - 246. ISSN 0270-9139

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Abstract

We have developed an in vitro system to study the interactions between biliary epithelium and lymphocytes using cultured human biliary epithelial cells. No class II antigens were detected by immunoperoxidase staining of the normal biliary epithelial cells, but alloactivated lymphocyte culture supernatants were able to induce class II expression. The activity of the supernatants was blocked with an anti‐γ‐interferon monoclonal antibody. In addition, recombinant human γ‐interferon alone induced the expression of class II antigens and increased the intensity of class I staining of cultured biliary epithelial cells. Biliary epithelial cell‐induced proliferation of alloreactive T lymphocytes demonstrated that the major histocompatibility complex molecules carry functional lymphocyteactivating determinants. The recognition of major histocompatibility complex determinants was confirmed by monoclonal antibody‐blocking studies and by stimulation of an alloreactive T‐cell clone. However, the biliary epithelial cells were much less potent stimulators than arterial endothelial cells tested in the same assay system. (HEPATOLOGY 1991;13:239–246). Copyright © 1991 American Association for the Study of Liver Diseases


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Saidman, SL
Duquesnoy, RJ
Zeevi, A
Fung, JJ
Starzl, TEtes11@pitt.eduTES11
Demetris, AJ
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1991
Date Type: Publication
Journal or Publication Title: Hepatology
Volume: 13
Number: 2
Page Range: 239 - 246
DOI or Unique Handle: 10.1002/hep.1840130207
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0270-9139
Other ID: uls-drl:31735062117779, Starzl CV No. 1226
Date Deposited: 08 Apr 2010 17:20
Last Modified: 13 Oct 2017 21:57
URI: http://d-scholarship.pitt.edu/id/eprint/4612

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