Saidman, SL and Duquesnoy, RJ and Zeevi, A and Fung, JJ and Starzl, TE and Demetris, AJ
(1991)
Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes.
Hepatology, 13 (2).
239 - 246.
ISSN 0270-9139
Abstract
We have developed an in vitro system to study the interactions between biliary epithelium and lymphocytes using cultured human biliary epithelial cells. No class II antigens were detected by immunoperoxidase staining of the normal biliary epithelial cells, but alloactivated lymphocyte culture supernatants were able to induce class II expression. The activity of the supernatants was blocked with an anti‐γ‐interferon monoclonal antibody. In addition, recombinant human γ‐interferon alone induced the expression of class II antigens and increased the intensity of class I staining of cultured biliary epithelial cells. Biliary epithelial cell‐induced proliferation of alloreactive T lymphocytes demonstrated that the major histocompatibility complex molecules carry functional lymphocyteactivating determinants. The recognition of major histocompatibility complex determinants was confirmed by monoclonal antibody‐blocking studies and by stimulation of an alloreactive T‐cell clone. However, the biliary epithelial cells were much less potent stimulators than arterial endothelial cells tested in the same assay system. (HEPATOLOGY 1991;13:239–246). Copyright © 1991 American Association for the Study of Liver Diseases
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Saidman, SL | | | | | Duquesnoy, RJ | | | | | Zeevi, A | | | | | Fung, JJ | | | | | Starzl, TE | tes11@pitt.edu | TES11 | | | Demetris, AJ | | | |
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| Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
| Date: |
1 January 1991 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Hepatology |
| Volume: |
13 |
| Number: |
2 |
| Page Range: |
239 - 246 |
| DOI or Unique Handle: |
10.1002/hep.1840130207 |
| Institution: |
University of Pittsburgh |
| Refereed: |
Yes |
| ISSN: |
0270-9139 |
| Other ID: |
uls-drl:31735062117779, Starzl CV No. 1226 |
| Date Deposited: |
08 Apr 2010 17:20 |
| Last Modified: |
21 Jan 2019 14:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/4612 |
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