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Identification of novel genes involved in telomere maintenance

Parikh, Anishka (2024) Identification of novel genes involved in telomere maintenance. Undergraduate Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Many proteins necessary for telomere maintenance and lengthening have been identified, but there are a host of proteins that have yet to be discovered in these processes. Identifying these telomere genes would be important in better understanding the telomere lengthening process and in understanding telomere mediated diseases such as pulmonary fibrosis. To do this, we created a forward genetic screen using CRISPR-Cas9 and a variant toxic TR sequence, AU5. Any cells with knockouts in genes important in telomere maintenance would be unable to add the AU5 sequence and therefore be able to tolerate the otherwise toxic sequence. We identified several genes that were able to survive the AU5 treatment, some of which were known telomere maintenance genes such as TERT and ACD. Further characterization and analysis of these genes could prove valuable in understanding telomere biology and potentially in understanding telomere mediated disease as well.

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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Parikh, Anishkaahp23@pitt.eduahp23
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAlder, Jonathanjalder@pitt.edu
Committee MemberBerman, Andreaajb190@pitt.edu
Committee MemberHainer, Sarahsarah.hainer@pitt.edu
Committee MemberBatista, Luislbatista@wustl.edu
Date: 22 April 2024
Date Type: Publication
Defense Date: 4 March 2024
Approval Date: 22 April 2024
Submission Date: 17 April 2024
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 61
Institution: University of Pittsburgh
Schools and Programs: David C. Frederick Honors College
Degree: BPhil - Bachelor of Philosophy
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: telomere, telomerase, CRISPR screen
Date Deposited: 22 Apr 2024 16:47
Last Modified: 22 Apr 2024 16:47
URI: http://d-scholarship.pitt.edu/id/eprint/46149

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