Parmar, Anand
(2024)
Age-related Comparison of Protein Composition in Serum and Platelet-Rich Fibrin (PRF).
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Periodontal disease is one of several inflammatory disease that is found with a higher prevalence in the older adult populations. The pro-inflammatory cytokines IL-6 and TNF- are two proteins that are found in higher concentrations in aging populations and may contribute to the pathogenesis of disease and healing alterations. Additionally, with healing alterations in this population we find a decrease in overall angiogenesis and regenerative potential with reduced delivery of growth factors. Without these growth factors present, conversion to healing phases maybe delayed or altered. Autologous blood products such as platelet-rich fibrin (PRF) are one way to improve wound healing and regeneration by delivering increased concentrations of growth factors. For the current study, growth factors VEGF and PDGF and pro-inflammatory cytokines IL-6 and TNF- were chosen to be analyzed as a function of age in PRF and serum preparations. 43 participants (22 younger and 21 older adults) were enrolled in the study and 20mL of blood was collected for protein quantification. No statistically significant difference in the PRF and whole serum preparations of growth factors were noted in the older adult population. However, cytokine concentrations were statistically significantly higher after initial release 2hrs (day 1). Plasminogen was tested to further understand the degradation of fibrin clots and was significantly higher in both PRF and whole serum preparations in the older adult participant group. Further clinical studies evaluating the age-related differences in growth factors, cytokines and plasminogen and their relationship to wound healing outcomes would be of benefit in this field.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
9 July 2024 |
| Date Type: |
Publication |
| Defense Date: |
6 June 2024 |
| Approval Date: |
9 July 2024 |
| Submission Date: |
7 June 2024 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
62 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Dental Medicine > Dental Science |
| Degree: |
MDS - Master of Dental Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
PRF, platelet-rich fibrin, protein composition, serum |
| Date Deposited: |
09 Jul 2024 13:25 |
| Last Modified: |
09 Jul 2024 13:25 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/46491 |
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