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Composition of human islet cell preparations for transplantation

Sever, CE and Demetris, AJ and Zeng, J and Carroll, P and Tzakis, A and Fung, JJ and Starzl, TE and Ricordi, C (1992) Composition of human islet cell preparations for transplantation. Acta Diabetologica, 28 (3-4). 233 - 238. ISSN 0940-5429

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Abstract

To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30-80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10-20%. After 1 week in culture the islet cell content of less highly purified isolates (30-40% islets) dropped dramatically to 5%. The highly purified isolates (70-80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment. © 1992 Springer-Verlag.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Sever, CE
Demetris, AJ
Zeng, J
Carroll, P
Tzakis, A
Fung, JJ
Starzl, TEtes11@pitt.eduTES11
Ricordi, C
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 September 1992
Date Type: Publication
Journal or Publication Title: Acta Diabetologica
Volume: 28
Number: 3-4
Page Range: 233 - 238
DOI or Unique Handle: 10.1007/bf00779005
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0940-5429
Other ID: uls-drl:31735062111632, Starzl CV No. 1370
Date Deposited: 08 Apr 2010 17:23
Last Modified: 13 Oct 2017 21:57
URI: http://d-scholarship.pitt.edu/id/eprint/4756

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