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Islet xenografts in fully xenogeneic (rat----mouse) chimeras: evidence for normal regulation of function in a xenogeneic mouse environment.

Ricordi, C and Zeng, Y and Carroll, PB and Rilo, HL and Beretier, DR and Starzl, TE and Ildstad, ST (1992) Islet xenografts in fully xenogeneic (rat----mouse) chimeras: evidence for normal regulation of function in a xenogeneic mouse environment. Surgery, 112 (2). 327 - 332. ISSN 0039-6060

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Abstract

BACKGROUND: Transplantation of untreated rat bone marrow into mouse recipients conditioned by total-body irradiation results in fully xenogeneic chimerism (rat----mouse). The chimerism is stable for up to 10 months, survival is excellent, and there is no evidence for graft-versus-host disease. We recently reported the long-term survival (greater than 180 days) of donor-specific pancreatic islet xenografts in these fully xenogeneic chimeras. METHODS: Chimeras were prepared and typed for chimerism at 6 weeks, and diabetes was induced by streptozocin injection. Donor-specific pancreatic islets were placed under the renal capsule and recipient blood glucose levels were followed biweekly. The aim of this study was to examine whether the transplanted pancreatic islets exhibited normal function in a xenogeneic environment and assess whether the islet xenografts were not only sufficient to support euglycemia but also regulated in function in response to a glucose challenge. RESULTS: We report for the first time that donor-specific rat islet xenografts were capable of producing normal basal and peak levels of insulin and responding to a glucose challenge in a manner similar to that of normal mouse islets. CONCLUSIONS: These data indicate that donor-specific rat islet xenografts are functional and regulated normally in fully xenogeneic (rat----mouse) chimeras.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ricordi, C
Zeng, Y
Carroll, PB
Rilo, HL
Beretier, DR
Starzl, TEtes11@pitt.eduTES11
Ildstad, ST
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: August 1992
Date Type: Publication
Journal or Publication Title: Surgery
Volume: 112
Number: 2
Page Range: 327 - 332
Institution: University of Pittsburgh
Refereed: Yes
Uncontrolled Keywords: Animals, Blood Glucose, Glucagon, Insulin, Islets of Langerhans, Islets of Langerhans Transplantation, Male, Mice, Mice, Inbred Strains, Rats, Rats, Inbred Strains, Reference Values, Transplantation, Heterologous
ISSN: 0039-6060
Funders: NIDDK NIH HHS (R01 DK029961-19), NIAID NIH HHS (AI30615), NIDDK NIH HHS (1RO1DK43901), NIDDK NIH HHS (DK29961)
Other ID: uls-drl:31735062111889, Starzl CV No. 1394
Date Deposited: 08 Apr 2010 17:23
Last Modified: 13 Oct 2017 21:56
URI: http://d-scholarship.pitt.edu/id/eprint/4780

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