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An improved technique for isolated perfusion of rat livers and an evaluation of perfusates

Mischinger, HJ and Walsh, TR and Liu, T and Rao, PN and Rubin, R and Nakamura, K and Todo, S and Starzl, TE (1992) An improved technique for isolated perfusion of rat livers and an evaluation of perfusates. Journal of Surgical Research, 53 (2). 158 - 165. ISSN 0022-4804

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Abstract

We have modified the apparatus for isolated rat liver perfusion (IPRL) in order to be able to perform two perfusions simultaneously. In addition, we studied the quality and stability of livers by comparison of five different perfusates: Blood (Group A), Original Krebs Henseleit buffer (Group B), Krebs buffer with glucose (Group C) or bovine serum albumin (BSA) added, (Group D). In a last group (E) albumin, glucose, and taurocholic acid were added to Krebs. After 180 min of perfusion, livers perfused with solutions including 2% albumin (Group D, E) had a significantly higher release of hepatocellular and endothelial cell (purine nucleoside phosphorylase) enzymes and lower bile production as compared to Groups A, B, and C (P < 0.0001). Increasing levels of purine nucleoside phosphorylase (PNP), a reflection of damage to the microvascular endothelium preceded the increases in hepatocellular enzymes. Histologically, damages of sinusoidal endothelial cells and hepatocytes are appreciated moderate to severe in Groups D and E, slight to mild in Groups A and B, and not significant in Group C. These results suggest that BSA may have toxic effects to the perfused rat liver. These data also confirm that the IPRL modified for simultaneous perfusion of two livers is efficient, and that with this technique the rat liver can be optimally perfused for up to 3 hr with oxygenated Krebs Henseleit buffer without additives (Group B) and without blood. These two improvements should allow those performing studies with perfused rat livers to obtain data in a more efficient, accurate, and inexpensive fashion. © 1992.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mischinger, HJ
Walsh, TR
Liu, T
Rao, PN
Rubin, R
Nakamura, K
Todo, S
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1992
Date Type: Publication
Journal or Publication Title: Journal of Surgical Research
Volume: 53
Number: 2
Page Range: 158 - 165
DOI or Unique Handle: 10.1016/0022-4804(92)90028-x
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0022-4804
Other ID: uls-drl:31735062112085, Starzl CV No. 1414
Date Deposited: 08 Apr 2010 17:24
Last Modified: 13 Oct 2017 21:58
URI: http://d-scholarship.pitt.edu/id/eprint/4800

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