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Preformed lymphocytotoxic antibodies: The effects of class, titer and specificity on liver vs. heart allografts

Furuya, T and Murase, N and Nakamura, K and Woo, J and Todo, S and Demetris, AJ and Starzl, TE (1992) Preformed lymphocytotoxic antibodies: The effects of class, titer and specificity on liver vs. heart allografts. Hepatology, 16 (6). 1415 - 1422. ISSN 0270-9139

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The effect on liver and heart allograft survival (ACI rats to Lewis rats) was studied after three methods of recipient presensitization and after different intervals between sensitization and transplantation. With comparable lymphocytotoxic antibody titers, liver allografts always survived longer than heart grafts. The titer, class and specificity of the antibodies varied with the method of sensitization. Four skin grafts produced IgG and IgM lymphocytotoxic antibody titers of 1:2,000 to 4,000. The IgG fraction was shown to have hepatic vascular endothelial specificity by indirect immunofluorescence. These primed recipients hyperacutely rejected both heart and liver allografts, which showed vascular deposition of IgG antibodies. Survival of either kind of graft was inversely proportional to the lymphocytotoxic antibody titer and length of time after placement of the last skin graft. Presensitization with a single heterotopic heart graft produced an even higher mixed IgG and IgM lymphocytotoxic antibody titer of 1:8,000 but with less IgG vascular endothelial specificity. These animals also hyperacutely rejected heart or liver grafts with tissue deposition of IgG but less consistently and with a weaker correlation with lymphocytotoxic antibody titers and time after sensitization. Sensitization with two pretransplant blood transfusions produced the lowest titer (1:500 to 1,000) and the least IgG vascular endothelial specificity. Liver allograft survival was routinely enhanced in these animals, and little effect was seen on heart grafts. Collectively, the experiments showed that the liver is not only resistant to antibody‐mediated rejection relative to the heart but is more easily enhanced. A more precise characterization of preformed antibodies may increase the ability to predict the outcome of liver transplantation in sensitized recipients or guide pretransplant strategies to foster enhancing antibodies. Copyright © 1992 American Association for the Study of Liver Diseases


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Furuya, T
Murase, N
Nakamura, K
Woo, J
Todo, S
Demetris, AJ
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1992
Date Type: Publication
Journal or Publication Title: Hepatology
Volume: 16
Number: 6
Page Range: 1415 - 1422
DOI or Unique Handle: 10.1002/hep.1840160618
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0270-9139
Other ID: uls-drl:31735062112416, Starzl CV No. 1454
Date Deposited: 08 Apr 2010 17:24
Last Modified: 27 Jan 2019 02:55


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