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Fk506 measurement: Comparison of different analytical methods

Warty, VS and Zuckerman, S and Venkataramanan, R and Lever, J and Fung, J and Starzl, T (1993) Fk506 measurement: Comparison of different analytical methods. Therapeutic Drug Monitoring, 15 (3). 204 - 208. ISSN 0163-4356

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Abstract

In this study, we used solid-phase extraction with Sep-Pak and a liquid-liquid extraction with methylene chloride as two primary methods of extracting FK506 from plasma. The extracts were either analyzed directly by enzyme-linked immunosorbent assay (ELISA) or subjected to high-performance liquid chromatography (HPLC) separation and different fractions were analyzed by ELISA. Serial blood samples were obtained from four kidney transplant patients and four patients who underwent liver transplantation, from day 1 until day 30-35 posttransplantation. There was no significant difference in the FK506 plasma concentration as measured by all four methods in normal transplant patients. However, in liver transplant patients, the solid-phase extraction method gave higher FK506 concentrations than the methylene chloride extraction during the early postoperative period. The concentrations measured after methylene chloride extraction were higher than that after HPLC-ELISA. This higher FK506 concentration measured by direct ELISA could be attributed to possible cross-reacting metabolites that were present in the plasma of patients with abnormal liver functions. Once liver function returns to normal, all four methods give identical plasma concentrations for FK506. © 1993 Raven Press, Ltd., New York.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Warty, VS
Zuckerman, S
Venkataramanan, Rrv@pitt.eduRV
Lever, J
Fung, J
Starzl, Ttes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1993
Date Type: Publication
Journal or Publication Title: Therapeutic Drug Monitoring
Volume: 15
Number: 3
Page Range: 204 - 208
DOI or Unique Handle: 10.1097/00007691-199306000-00005
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0163-4356
Other ID: uls-drl:31735062113224, Starzl CV No. 1533
Date Deposited: 08 Apr 2010 17:26
Last Modified: 02 Feb 2019 14:56
URI: http://d-scholarship.pitt.edu/id/eprint/4919

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