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Induction of donor-specific transplantation tolerance to skin and cardiac allografts using mixed chimerism in (A + B → A) in rats

Markus, PM and Selvaggi, G and Cai, X and Fung, JJ and Starzl, TE (1993) Induction of donor-specific transplantation tolerance to skin and cardiac allografts using mixed chimerism in (A + B → A) in rats. Cell Transplantation, 2 (4). 345 - 353. ISSN 0963-6897

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Abstract

Mixed allogeneic chimerism (A + B → A) was induced in rats by reconstitution of lethally irradiated LEW recipients with a mixture of T-cell depleted (TCD) syngeneic and TCD allogeneic ACI bone marrow. Thirty-seven percent of animals repopulated as stable mixed lymphopoietic chimeras, while the remainder had no detectable allogeneic chimerism. When evaluated for evidence of donor-specific transplantation tolerance, only those recipients with detectable allogeneic lymphoid chimerism exhibited acceptance of donor-specific skin and cardiac allografts. Despite transplantation over a major histocompatibility complex (MHC)- and minor-disparate barrier, animals accepted donor-specific ACI skin and primarily vascularized cardiac allografts permanently, while rejecting third party Brown Norway (BN) grafts. The tolerance induced was also donor-specific in vitro as evidenced by specific hyporeactivity to the allogeneic donor lymphoid elements, yet normal reactivity to MHC-disparate third party rat lymphoid cells. This model for mixed chimerism in the rat will be advantageous to investigate specific transplantation tolerance to primarily vascularized solid organ grafts that can be performed with relative ease in the rat, but not in the mouse, and may provide a method to study the potential existence of organ- or tissue-specific alloantigens in primarily vascularized solid organ allografts. © 1993.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Markus, PM
Selvaggi, G
Cai, X
Fung, JJ
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 July 1993
Date Type: Publication
Journal or Publication Title: Cell Transplantation
Volume: 2
Number: 4
Page Range: 345 - 353
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0963-6897
Other ID: uls-drl:31735062124866, Starzl CV No. 1601
Date Deposited: 08 Apr 2010 17:27
Last Modified: 13 Oct 2017 23:55
URI: http://d-scholarship.pitt.edu/id/eprint/4987

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