Interferon-γ induced expression of MHC antigens facilitates identification of donor cells in chimeric transplant recipients

Rao, AS and Demetris, AJ and Qian, S and Murase, N and Li, Y and Fung, JJ and Starzl, TE (1994) Interferon-γ induced expression of MHC antigens facilitates identification of donor cells in chimeric transplant recipients. Cell Transplantation, 3 (4). 345 - 348. ISSN 0963-6897

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Abstract

After whole organ transplantation, donor bone marrow-derived cells migrate out of the graft into the recipient, leading to establishment of chimerism, which is the first step towards the subsequent induction of donor-specific tolerance. In routine immunohistochemical staining, monoclonal antibodies specific for heterotopic MHC alleles are used to identify donor and recipient cells. However, it is difficult to detect these cells using this technique in long-term allograft recipients who have a persistently low donor cell population (microchimerism). Because Interferon-gamma (IFN-γ) is known to induce expression of MHC class I and class II cell surface molecules, we used this cytokine 12-48 h before sacrifice, to facilitate the identification of donor and recipient cells in the tissues of animals transplanted with either liver (B10 → C3H) or bone marrow (LEW → BN). In long-term allograft recipients, the use of IFN-γ for as briefly as 12 h prior to sacrifice, results in marked upregulation of class I and class II antigens, leading to easy identification of ubiquitously distributed low numbers of donor cells. © 1994.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Rao, AS Demetris, AJ Qian, S Murase, N Li, Y Fung, JJ Starzl, TE tes11@pitt.edu TES11
Centers:	Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date:	1 January 1994
Date Type:	Publication
Journal or Publication Title:	Cell Transplantation
Volume:	3
Number:	4
Page Range:	345 - 348
DOI or Unique Handle:	10.1177/096368979400300412
Institution:	University of Pittsburgh
Refereed:	Yes
ISSN:	0963-6897
Other ID:	uls-drl:31735062125772, Starzl CV No. 1638
Date Deposited:	08 Apr 2010 17:27
Last Modified:	25 Jan 2019 23:55
URI:	http://d-scholarship.pitt.edu/id/eprint/5024

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