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Further steps of hepatic stimulatory substance purification

Francavilla, A and Barone, M and Van Thiel, DH and Mazzaferro, V and Prelich, JG and Starzl, TE (1991) Further steps of hepatic stimulatory substance purification. Digestive Diseases and Sciences, 36 (5). 674 - 680. ISSN 0163-2116

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The hepatic stimulatory substance (HSS) extracted from weanling rat livers was purified 381,000-fold using chromatographic techniques including nondissociating polyacrylamide gel electrophoresis (nondenaturing PAGE). The activity of this highly purified HSS, named Acr-F4, was assessed in two in vivo models. In 40% hepatectomized rats, it produced a fivefold increase in the proliferative rate normally seen following this partial hepatectomy. In Eck fistula dogs, the level of base increase in hepatocyte renewal was amplified threefold by an infusion of Acr-F4 (50 ng/kg/day). Acr-F4 had no influence on the regenerative response of the kidney following a unilateral nephrectomy or of the bowel following a 40% resection of the small bowel. On the basis of these findings, it can be concluded that HSS (Acr-F4) has a high biological activity and is organ specific. © 1991 Plenum Publishing Corporation.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Francavilla, A
Barone, M
Van Thiel, DH
Mazzaferro, V
Prelich, JG
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 May 1991
Date Type: Publication
Journal or Publication Title: Digestive Diseases and Sciences
Volume: 36
Number: 5
Page Range: 674 - 680
DOI or Unique Handle: 10.1007/bf01297037
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0163-2116
Other ID: uls-drl:31735062124973, Starzl CV No. 1643
Date Deposited: 08 Apr 2010 17:27
Last Modified: 22 Jun 2021 10:55


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