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Indefinite survival of rat islet allografts following infusion of donor bone marrow without cytoablation

Ricordi, C and Murase, N and Rastellini, C and Behboo, R and Demetris, AJ and Starzl, TE (1996) Indefinite survival of rat islet allografts following infusion of donor bone marrow without cytoablation. Cell Transplantation, 5 (1). 53 - 55. ISSN 0963-6897

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Abstract

We have tested the effect of donor bone marrow cell (DBMC) infusion on the survival of pancreatic islet allografts in the rat, without the use of cytoablative recipient conditioning. Lewis and diabetic Brown Norway rats were used as donors and recipients, respectively. Donor islets were placed beneath the left renal capsule. Infusion of DBMC and temporary immunosuppression followed by delayed islet transplantation resulted in indefinite survival of all islet grafts (MST > 180 days). Control animals demonstrated recurrent hyperglycemia (islet allografts rejection). Donor bone marrow derived cells were detected in the spleen and cervical lymph nodes of BN recipients of LEW bone marrow but not in the recipients of islet transplants alone. Second set full thickness skin grafts were performed in normal BN and in recipients of a previously successful ITX. Donor specific skin grafts were accepted in the animals that had received DBMC 40 days before the islet allograft, while animals receiving DBMC at the time of the islet allograft rejected the donor specific skin graft similarly to the controls. However, these animals did not reject a second set donor-specific islet transplant. The results indicate that radiation conditioning of the recipients was not necessary to induce microchimerism and graft acceptance in this rodent model of islet allotransplantation.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ricordi, C
Murase, N
Rastellini, C
Behboo, R
Demetris, AJ
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1996
Date Type: Publication
Journal or Publication Title: Cell Transplantation
Volume: 5
Number: 1
Page Range: 53 - 55
DOI or Unique Handle: 10.1016/0963-6897(95)02017-9
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0963-6897
Other ID: uls-drl:31735062133321, Starzl CV No. 1868
Date Deposited: 08 Apr 2010 17:31
Last Modified: 22 Oct 2017 01:55
URI: http://d-scholarship.pitt.edu/id/eprint/5254

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