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Effect of ischemia on the canine large bowel: A comparison with the small intestine

Takeyoshi, I and Zhang, S and Nakamura, K and Ikoma, A and Zhu, Y and Starzl, TE and Todo, S (1996) Effect of ischemia on the canine large bowel: A comparison with the small intestine. Journal of Surgical Research, 62 (1). 41 - 48. ISSN 0022-4804

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Abstract

Mucosal injury caused by ischemia and reperfusion has been well documented with the small intestine, but little is known about the colon. In the present study, the effect of warm and cold ischemia on the canine colon was studied and compared to that on the small intestine. After in situ flushing, the small intestine and the colon from six beagle dogs were removed and stored for 0.5, 1.5, and 3 hr at 37°C (warm ischemia) or for 1, 6, 12, 24, 36, and 48 hr at 4°C (cold ischemia). Electrophysiology, permeability, biochemistry, and histopathology of the specimens at each ischemic period and after reperfusion in the Ussing chamber were determined. Warm and cold ischemia induced duration-dependent suppression of electrophysiology in both organs, but the colonic mucosa retained higher activity of absorptive enterocytes and cryptic cells than the small intestine. Only the colon showed increased permeability of FITC-conjugated Dextran from the mucosal surface to the submucosal layer after prolonged ischemia. Changes in adenine nucleotides and purine catabolites were not markedly different between the organs. Histopathologic abnormalities during ischemia and after reperfusion were more serious with the small intestine than with the colon. Compared to warm ischemia, hypothermia lessened or delayed these morphofunctional derangements in both organs, which became universally worsened after reperfusion. Colonic mucosa receives morphofunctional derangements from ischemia and reperfusion, but the severity of the damage was much less severe in the colon than in the small intestine.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Takeyoshi, I
Zhang, S
Nakamura, K
Ikoma, A
Zhu, Y
Starzl, TEtes11@pitt.eduTES11
Todo, S
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1996
Date Type: Publication
Journal or Publication Title: Journal of Surgical Research
Volume: 62
Number: 1
Page Range: 41 - 48
DOI or Unique Handle: 10.1006/jsre.1996.0170
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0022-4804
Other ID: uls-drl:31735062133479, Starzl CV No. 1883
Date Deposited: 08 Apr 2010 17:32
Last Modified: 16 Oct 2017 21:55
URI: http://d-scholarship.pitt.edu/id/eprint/5269

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