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Intestinal neuromuscular function after preservation and transplantation

Hamada, N and Hutson, WR and Nakada, K and Ikoma, A and Suzuki, T and Zhu, Y and Starzl, TE and Todo, S (1996) Intestinal neuromuscular function after preservation and transplantation. Journal of Surgical Research, 63 (2). 460 - 466. ISSN 0022-4804

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Abstract

While it is well known that prolonged preservation of the intestinal graft causes severe mucosal damage after transplantation, little is known about the effect on neuromuscular function. The entire small intestine of adult hound dogs was flushed and preserved with cold lactated Ringer's solution and autotransplanted either immediately (n = 6) or after 24 hr (n = 6). Animals undergoing sham operation (n = 4) were used as a control. Fasting motility and the response of the intestinal smooth muscle and enteric nerves to bethanechol (100 μg/kg/0.5 hr, iv) and cisapride (0.5 mg/kg, iv) were determined by a multiple strain gauge method on Postoperative Days 2, 4, 7, 14, 21, and 28. Compared to the control, immediately transplanted grafts and those preserved for 24 hr developed delayed reappearance of migrating myoelectric complexes (MMC), hypercontractile activity, and reduced response to bethanechol and cisapride administration. Animals in the preservation group developed more abnormal fasting motility after transplantation, but responses to bethanechol and cisapride stimulation were not markedly different from those of the immediate group. The reappearance of MMC occurred 3 weeks postoperatively in the preservation group compared to 2 days in the immediate group. The results of our study indicate that intestinal dysmotility is augmented in prolonged-preservation grafts compared to those with brief preservation. The dysmotility was transient and normalized 3 to 4 weeks after surgery. Preservation and reperfusion injury to the neuromuscular system of intestinal grafts are reversible and are attenuated by simple hypothermia.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hamada, N
Hutson, WR
Nakada, K
Ikoma, A
Suzuki, T
Zhu, Y
Starzl, TEtes11@pitt.eduTES11
Todo, S
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 July 1996
Date Type: Publication
Journal or Publication Title: Journal of Surgical Research
Volume: 63
Number: 2
Page Range: 460 - 466
DOI or Unique Handle: 10.1006/jsre.1996.0293
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0022-4804
Other ID: uls-drl:31735062133586, Starzl CV No. 1893
Date Deposited: 08 Apr 2010 17:32
Last Modified: 13 Oct 2017 21:55
URI: http://d-scholarship.pitt.edu/id/eprint/5279

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