Qian, S and Fu, F and Li, Y and Lu, L and Rao, AS and Starzl, TE and Thomson, AW and Fung, JJ
(1996)
Impact of donor MHC class I or class II antigen deficiency on first- and second-set rejection of mouse heart or liver allografts.
Immunology, 88 (1).
124 - 129.
ISSN 0019-2805
Abstract
The influence of donor major histocompatibility complex (MHC) class I- or class II-deficiency on the initiation of first- and second-set rejection of mouse heart and liver allografts was examined. C3H (H-2(k)) mice received heterotopic cardiac or orthotopic liver grafts from unmodified B 10 (H-2b), B6 (H-2b), b2m (H-2b; class I deficient) or AB0 (H-2b; class II deficient) donors. Organ survival was also investigated in C3H recipients that had been presensitized by a normal B10 skin graft 2-3 weeks before heart or liver transplantation. The absence of cell surface MHC class I or class II resulted in significant prolongation of primary cardiac allograft survival. Three of seven (43%) MHC class I-deficient, and two of five (40%) class II-deficient heart grafts were accepted indefinitely (survival time > 100 days). Thus both MHC class I and class II molecules appear to be important for the elicitation of first-set rejection in the heart allograft model. All liver allografts survived > 100 days in normal recipients. In C3H recipients that had been presensitized by a B10 skin graft, however, both heart and liver grafts from AB0 (class II deficient) donors underwent accelerated rejection (median survival time [MST] 3 and 4 days. respectively). In contrast, liver grafts from class I-deficient mice (b2m) were still accepted indefinitely by B10 skin-presensitized C3H recipients, whereas class I-deficient hearts survived significantly longer than those from class II-deficient or normal donors. These data demonstrate that the expression of donor MHC class I, and not class II is crucial in initiating second-set organ allograft rejection. In vitro monitoring revealed that at the time of organ transplant, both splenocvtes and serum of the skin-presensitized animals displayed high cytotoxicity against AB(o) (class II-deficient) but not against b2m (class I-deficient) targets.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Qian, S | | | | | Fu, F | | | | | Li, Y | | | | | Lu, L | | | | | Rao, AS | | | | | Starzl, TE | tes11@pitt.edu | TES11 | | | Thomson, AW | | | | | Fung, JJ | | | |
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| Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
| Date: |
1 January 1996 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Immunology |
| Volume: |
88 |
| Number: |
1 |
| Page Range: |
124 - 129 |
| DOI or Unique Handle: |
10.1046/j.1365-2567.1996.d01-633.x |
| Institution: |
University of Pittsburgh |
| Refereed: |
Yes |
| ISSN: |
0019-2805 |
| Other ID: |
uls-drl:31735062126960, Starzl CV No. 1974 |
| Date Deposited: |
08 Apr 2010 17:33 |
| Last Modified: |
11 Jun 2021 22:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/5360 |
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