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Impact of donor MHC class I or class II antigen deficiency on first- and second-set rejection of mouse heart or liver allografts

Qian, S and Fu, F and Li, Y and Lu, L and Rao, AS and Starzl, TE and Thomson, AW and Fung, JJ (1996) Impact of donor MHC class I or class II antigen deficiency on first- and second-set rejection of mouse heart or liver allografts. Immunology, 88 (1). 124 - 129. ISSN 0019-2805

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Abstract

The influence of donor major histocompatibility complex (MHC) class I- or class II-deficiency on the initiation of first- and second-set rejection of mouse heart and liver allografts was examined. C3H (H-2(k)) mice received heterotopic cardiac or orthotopic liver grafts from unmodified B 10 (H-2 b ), B6 (H-2 b ), b2m (H-2 b ; class I deficient) or AB 0 (H-2 b ; class II deficient) donors. Organ survival was also investigated in C3H recipients that had been presensitized by a normal B10 skin graft 2-3 weeks before heart or liver transplantation. The absence of cell surface MHC class I or class II resulted in significant prolongation of primary cardiac allograft survival. Three of seven (43%) MHC class I-deficient, and two of five (40%) class II-deficient heart grafts were accepted indefinitely (survival time > 100 days). Thus both MHC class I and class II molecules appear to be important for the elicitation of first-set rejection in the heart allograft model. All liver allografts survived > 100 days in normal recipients. In C3H recipients that had been presensitized by a B10 skin graft, however, both heart and liver grafts from AB 0 (class II deficient) donors underwent accelerated rejection (median survival time [MST] 3 and 4 days. respectively). In contrast, liver grafts from class I-deficient mice (b2m) were still accepted indefinitely by B10 skin-presensitized C3H recipients, whereas class I-deficient hearts survived significantly longer than those from class II-deficient or normal donors. These data demonstrate that the expression of donor MHC class I, and not class II is crucial in initiating second-set organ allograft rejection. In vitro monitoring revealed that at the time of organ transplant, both splenocvtes and serum of the skin-presensitized animals displayed high cytotoxicity against AB(o) (class II-deficient) but not against b2m (class I-deficient) targets.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Qian, S
Fu, F
Li, Y
Lu, L
Rao, AS
Starzl, TEtes11@pitt.eduTES11
Thomson, AW
Fung, JJ
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 20 May 1996
Date Type: Publication
Journal or Publication Title: Immunology
Volume: 88
Number: 1
Page Range: 124 - 129
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0019-2805
Other ID: uls-drl:31735062126960, Starzl CV No. 1974
Date Deposited: 08 Apr 2010 17:33
Last Modified: 13 Oct 2017 22:56
URI: http://d-scholarship.pitt.edu/id/eprint/5360

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