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Evidence for engraftment of human bone marrow cells in non-lethally irradiated baboons

Fontes, P and Rogers, J and Rao, AS and Trucco, M and Zeevi, A and Ricordi, C and Fung, JJ and Starzl, TE (1997) Evidence for engraftment of human bone marrow cells in non-lethally irradiated baboons. Transplantation, 64 (11). 1595 - 1698. ISSN 0041-1337

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Abstract

Background. Prior to organ harvesting, an attempt was made to modulate the donor's immune responses against prospective xenogeneic recipients by infusion of 'recipient-type' bone marrow. Methods. For this purpose, baboons conditioned with total lymphoid irradiation were given 6x108 unmodified human bone marrow cells/kg body weight with no subsequent treatment. Results. Animals survived until they were euthanized at 18 months. Using primers specific for human chorionic gonadotrophin gene, the presence of human DNA was confirmed by polymerase chain reaction in the blood of one animal for up to 18 months after cell transplantation; in the other animal, xenogeneic chimerism became undetectable in the blood at 6 months after bone marrow infusion. However, tissue samples obtained from both animals at the time they were euthanized bad evidence of donor (human) DNA. Additionally, the presence of donor DNA in individually harvested colonies of erythroid and myeloid lineages suggested that infused human bone marrow cells had engrafted across the xenogeneic barrier in both baboons. Conclusions. Bone marrow transplantation from human to baboon leads to establishment of chimerism and modulation of donor-specific immune reactivity, which suggests that this strategy could be reproducibly employed to crease 'surrogate' tolerogenesis in prospective donors for subsequent organ transplantation across xenogeneic barriers.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Fontes, P
Rogers, J
Rao, AS
Trucco, Mmnt@pitt.eduMNT
Zeevi, A
Ricordi, C
Fung, JJ
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 15 December 1997
Date Type: Publication
Journal or Publication Title: Transplantation
Volume: 64
Number: 11
Page Range: 1595 - 1698
DOI or Unique Handle: 10.1097/00007890-199712150-00016
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0041-1337
Other ID: uls-drl:31735062127174, Starzl CV No. 1995
Date Deposited: 08 Apr 2010 17:34
Last Modified: 04 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/5381

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