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Augmenter of liver regeneration enhances the success rate of fetal pancreas transplantation in rodents

Adams, GA and Maestri, M and Squiers, EC and Alfrey, EJ and Starzl, TE and Dafoe, DC (1998) Augmenter of liver regeneration enhances the success rate of fetal pancreas transplantation in rodents. Transplantation, 65 (1). 32 - 36. ISSN 0041-1337

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Abstract

Background. Treatment of fetal pancreas (FP) isografts with insulin- like growth factor-I greatly improves the rate of conversion to euglycemia in diabetic rats. Complete knowledge of other factors that may facilitate the engraftment and function of FP in vivo is still embryonic. Augmenter of liver regeneration (ALR) is a newly described polypeptide growth factor found in weanling rat livers. ALR has trophic effects on regenerating liver. We studied the effects of in situ administration of this agent on FP isografts in rats. Methods. Streptozotocin-diabetic Lewis rats (blood glucose > 300 mg/dl) received 16 FP isografts transplanted intramuscularly. ALR was delivered from day 1 through day 14, in doses of 40 or 400 ng/kg/d. Animals were followed for 3 months with serial weights and blood glucose monitoring. These animals were compared with those treated with vehicle alone. Results. Of the group treated with ALR at 40 ng/kg/day for 14 days, 89% (eight of nine) were euglycemic (P=0.0003). Of the group treated with ALR at 400 ng/kg/day for 14 days, 88% (seven of eight) were euglycemic (P=0.0007). Of the group treated with vehicle alone, none of the six were euglycemic. Euglycemia is defined here as glucose < 200 mg/dl for 3 days. Pathology of the intramuscular transplant site showed patches of islet tissue embedded in fat. These patches demonstrated insulin immunoreactivity. Conclusions. Diabetes was reversed in a significantly greater proportion of FP + ALR-treated recipients than those animals treated with vehicle alone. Local delivery of growth factors my be used as an adjunct to FP transplantation to improve the rate of success. This in situ model may be useful to further evaluate other soluble factors.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Adams, GA
Maestri, M
Squiers, EC
Alfrey, EJ
Starzl, TEtes11@pitt.eduTES11
Dafoe, DC
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 15 January 1998
Date Type: Publication
Journal or Publication Title: Transplantation
Volume: 65
Number: 1
Page Range: 32 - 36
DOI or Unique Handle: 10.1097/00007890-199801150-00007
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0041-1337
Other ID: uls-drl:31735062127398, Starzl CV No. 2018
Date Deposited: 08 Apr 2010 17:34
Last Modified: 30 Oct 2017 23:56
URI: http://d-scholarship.pitt.edu/id/eprint/5404

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