Kawashima, Y and Takeyoshi, I and Furukawa, H and Lee, RG and Starzl, TE and Todo, S
(1999)
Cold preservation of the human colon and ileum with University of Wisconsin solution.
Clinical Transplantation, 13 (5).
420 - 425.
ISSN 0902-0063
Abstract
The inclusion of the colon in the intestinal graft resulted in worsening patient and graft outcome and increased the incidence of infection and rejection. In this study, we examine the role of ischemia on the barrier function of the epithelium during cold ischemia. Samples were collected from 15 harvested and transplanted human donor grafts (colon, 10; ileum, 6), which were immersed in University of Wisconsin (UW) solution. Ischemia (6, 12, 24, and 45 h) and reoxygenation were performed to evaluate the mucosal electrical status using the Ussing chamber technique. The functions of enterocytes and crypt cells were tested by glucose and theophylline challenge. Modified Park's classification was applied to evaluate the severity of mucosal damage under light microscopy. The colon had higher levels of baseline potential difference, short-circuit current, and resistance than the ileum during 6-48 h of ischemia. Colonic epithelial cells responded well to theophylline stimulation at 24 h of ischemia, while there was no ileal response. The colonic mucosa was histopathologically well preserved in UW solution for 48 h, and mucosal damage induced by reoxygenation was less than in the ileum. In conclusion, electrophysiologically and histopathologically, the colon is less susceptible to cold preservation damage than the ileum during storage with UW solution.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
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Kawashima, Y | | | | Takeyoshi, I | | | | Furukawa, H | | | | Lee, RG | | | | Starzl, TE | tes11@pitt.edu | TES11 | | Todo, S | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
Date: |
13 October 1999 |
Date Type: |
Publication |
Journal or Publication Title: |
Clinical Transplantation |
Volume: |
13 |
Number: |
5 |
Page Range: |
420 - 425 |
DOI or Unique Handle: |
10.1034/j.1399-0012.1999.130508.x |
Institution: |
University of Pittsburgh |
Refereed: |
Yes |
ISSN: |
0902-0063 |
Other ID: |
uls-drl:31735062120245, Starzl CV No. 2112 |
Date Deposited: |
08 Apr 2010 17:36 |
Last Modified: |
02 Feb 2019 13:56 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/5498 |
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