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Cold preservation of the human colon and ileum with University of Wisconsin solution

Kawashima, Y and Takeyoshi, I and Furukawa, H and Lee, RG and Starzl, TE and Todo, S (1999) Cold preservation of the human colon and ileum with University of Wisconsin solution. Clinical Transplantation, 13 (5). 420 - 425. ISSN 0902-0063

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Abstract

The inclusion of the colon in the intestinal graft resulted in worsening patient and graft outcome and increased the incidence of infection and rejection. In this study, we examine the role of ischemia on the barrier function of the epithelium during cold ischemia. Samples were collected from 15 harvested and transplanted human donor grafts (colon, 10; ileum, 6), which were immersed in University of Wisconsin (UW) solution. Ischemia (6, 12, 24, and 45 h) and reoxygenation were performed to evaluate the mucosal electrical status using the Ussing chamber technique. The functions of enterocytes and crypt cells were tested by glucose and theophylline challenge. Modified Park's classification was applied to evaluate the severity of mucosal damage under light microscopy. The colon had higher levels of baseline potential difference, short-circuit current, and resistance than the ileum during 6-48 h of ischemia. Colonic epithelial cells responded well to theophylline stimulation at 24 h of ischemia, while there was no ileal response. The colonic mucosa was histopathologically well preserved in UW solution for 48 h, and mucosal damage induced by reoxygenation was less than in the ileum. In conclusion, electrophysiologically and histopathologically, the colon is less susceptible to cold preservation damage than the ileum during storage with UW solution.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Kawashima, Y
Takeyoshi, I
Furukawa, H
Lee, RG
Starzl, TEtes11@pitt.eduTES11
Todo, S
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 13 October 1999
Date Type: Publication
Journal or Publication Title: Clinical Transplantation
Volume: 13
Number: 5
Page Range: 420 - 425
DOI or Unique Handle: 10.1034/j.1399-0012.1999.130508.x
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0902-0063
Other ID: uls-drl:31735062120245, Starzl CV No. 2112
Date Deposited: 08 Apr 2010 17:36
Last Modified: 05 Dec 2017 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/5498

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