Nalesnik, MA and Zeevi, A and Randhawa, PS and Faro, A and Spichty, KJ and Demetris, AJ and Fung, JJ and Whiteside, TL and Starzl, TE
(1999)
Cytokine mRNA profiles in Epstein-Barr virus-associated post-transplant lymphoproliferative disorders.
Clin Transplant, 13 (1 Pt 1).
39 - 44.
ISSN 0902-0063
Abstract
Cytokine mRNA patterns were analyzed in 11 post-transplant lymphoproliferative disorder (PTLD) specimens using qualitative reverse-transcriptase polymerase chain reaction (RT-PCR). In each case, a pattern of IL2-, IFN gamma-, IL4+, IL10+ was seen. A similar pattern was observed in a spleen sample from 1 patient with contemporaneous PTLD elsewhere. Semiquantitative RT-PCR for cytokine message was performed using RNA from bronchoalveolar lavage (BAL) specimens obtained from 2 patients with pulmonary PTLD. In both cases, IL4 message predominated. Reduction of message coincided with resolution of the tumors. The pattern differed from that seen in 1 patient with acute pulmonary rejection, in which RT-PCR of BAL cells showed predominance of IL6 and IFN gamma. We conclude that at least some PTLDs exist within a T-helper cell type 2 (Th2)-like cytokine microenvironment. The presence of a similar mRNA pattern in an extratumoral specimen at the time of PTLD suggests that it may reflect a systemic phenomenon. Disappearance of this pattern following PTLD resolution indicates its dynamic nature and is consistent with the hypothesis that specific cytokines contribute to the development of PTLDs.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
---|
Nalesnik, MA | | | | Zeevi, A | | | | Randhawa, PS | | | | Faro, A | | | | Spichty, KJ | | | | Demetris, AJ | | | | Fung, JJ | | | | Whiteside, TL | | | | Starzl, TE | tes11@pitt.edu | TES11 | |
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Centers: |
Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute |
Date: |
February 1999 |
Date Type: |
Publication |
Journal or Publication Title: |
Clin Transplant |
Volume: |
13 |
Number: |
1 Pt 1 |
Page Range: |
39 - 44 |
DOI or Unique Handle: |
10.1034/j.1399-0012.1999.t01-2-130106.x |
Institution: |
University of Pittsburgh |
Refereed: |
Yes |
Uncontrolled Keywords: |
Bronchoalveolar Lavage Fluid, Cytokines, Epstein-Barr Virus Infections, Humans, Interferon-gamma, Interleukin-10, Interleukin-2, Interleukin-4, Lymphoma, Lymphoproliferative Disorders, Organ Transplantation, Polymerase Chain Reaction, RNA, Messenger |
ISSN: |
0902-0063 |
Funders: |
NIAAA NIH HHS (A-3176), NCI NIH HHS (P01-CA 47445) |
Other ID: |
uls-drl:31735062120344, Starzl CV No. 2126 |
Date Deposited: |
08 Apr 2010 17:36 |
Last Modified: |
31 Dec 2019 15:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/5512 |
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