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Cytokine mRNA profiles in Epstein-Barr virus-associated post-transplant lymphoproliferative disorders.

Nalesnik, MA and Zeevi, A and Randhawa, PS and Faro, A and Spichty, KJ and Demetris, AJ and Fung, JJ and Whiteside, TL and Starzl, TE (1999) Cytokine mRNA profiles in Epstein-Barr virus-associated post-transplant lymphoproliferative disorders. Clin Transplant, 13 (1 Pt 1). 39 - 44. ISSN 0902-0063

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Abstract

Cytokine mRNA patterns were analyzed in 11 post-transplant lymphoproliferative disorder (PTLD) specimens using qualitative reverse-transcriptase polymerase chain reaction (RT-PCR). In each case, a pattern of IL2-, IFN gamma-, IL4+, IL10+ was seen. A similar pattern was observed in a spleen sample from 1 patient with contemporaneous PTLD elsewhere. Semiquantitative RT-PCR for cytokine message was performed using RNA from bronchoalveolar lavage (BAL) specimens obtained from 2 patients with pulmonary PTLD. In both cases, IL4 message predominated. Reduction of message coincided with resolution of the tumors. The pattern differed from that seen in 1 patient with acute pulmonary rejection, in which RT-PCR of BAL cells showed predominance of IL6 and IFN gamma. We conclude that at least some PTLDs exist within a T-helper cell type 2 (Th2)-like cytokine microenvironment. The presence of a similar mRNA pattern in an extratumoral specimen at the time of PTLD suggests that it may reflect a systemic phenomenon. Disappearance of this pattern following PTLD resolution indicates its dynamic nature and is consistent with the hypothesis that specific cytokines contribute to the development of PTLDs.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Nalesnik, MA
Zeevi, A
Randhawa, PS
Faro, A
Spichty, KJ
Demetris, AJ
Fung, JJ
Whiteside, TL
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: February 1999
Date Type: Publication
Journal or Publication Title: Clin Transplant
Volume: 13
Number: 1 Pt 1
Page Range: 39 - 44
DOI or Unique Handle: 10.1034/j.1399-0012.1999.t01-2-130106.x
Institution: University of Pittsburgh
Refereed: Yes
Uncontrolled Keywords: Bronchoalveolar Lavage Fluid, Cytokines, Epstein-Barr Virus Infections, Humans, Interferon-gamma, Interleukin-10, Interleukin-2, Interleukin-4, Lymphoma, Lymphoproliferative Disorders, Organ Transplantation, Polymerase Chain Reaction, RNA, Messenger
ISSN: 0902-0063
Funders: NIAAA NIH HHS (A-3176), NCI NIH HHS (P01-CA 47445)
Other ID: uls-drl:31735062120344, Starzl CV No. 2126
Date Deposited: 08 Apr 2010 17:36
Last Modified: 31 Dec 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/5512

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