Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Simultaneous bone marrow and intestine transplantation promotes marrow-derived hematopoietic stem cell engraftment and chimerism

Nakao, A and Toyokawa, H and Kimizuka, K and Nalesnik, MA and Nozaki, I and Bailey, RJ and Demetris, AJ and Starzl, TE and Murase, N (2006) Simultaneous bone marrow and intestine transplantation promotes marrow-derived hematopoietic stem cell engraftment and chimerism. Blood, 108 (4). 1413 - 1420. ISSN 0006-4971

[img]
Preview
PDF
Accepted Version
Available under License : See the attached license file.

Download (1MB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

Organ allografts have been shown to provide a syngeneic microenvironment for organ-based donor hematopoietic stem cells to maintain long-lasting chimerism after transplantation. We hypothesized that organ allografts would also support engraftment and hematopoiesis of adjunctively infused donor marrow stem cells, syngeneic to organ grafts, in nonmyeloablated recipients. In BN-to-LEW and GFP-to-ACI rat combinations, donor bone marrow (BM) infusion together with small intestine transplantation (SITx) under short-course tacrolimus immunosuppression resulted in persistent macrochimerism (more than 5%) for 150 days. In contrast, after BM infusion or SITx alone, chimerism was temporary and disappeared by day 100. Y-chromosome polymerase chain reaction (PCR) in sex-mismatched male BM plus female intestine or female BM plus male intestine transplantation into female recipients suggested that persistent macrochimerism was derived from infused BM. BM infusion together with lymphoid-depleted intestine grafts also supported macrochimerism development; however, third-party intestine grafts did not. After GFP-positive BM plus wild-type (WT) SITx into ACI, large numbers of GFP-positive leukocytes were found in WT intestine grafts. Isolated cells from WT intestine grafts developed GFP-positive CFU-Cs and propagated multilineage GFP-positive leukocytes when adoptively transferred into lethally irradiated WT recipients. These findings suggest that intestine allograft supports simultaneously infused donor (syngeneic to organ grafts) marrow stem cell engraftment, differentiation, and persistence of chimerism. © 2006 by The American Society of H ematology.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Nakao, A
Toyokawa, H
Kimizuka, K
Nalesnik, MA
Nozaki, I
Bailey, RJ
Demetris, AJ
Starzl, TEtes11@pitt.eduTES11
Murase, N
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 15 August 2006
Date Type: Publication
Journal or Publication Title: Blood
Volume: 108
Number: 4
Page Range: 1413 - 1420
DOI or Unique Handle: 10.1182/blood-2006-02-004341
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0006-4971
Other ID: uls-drl:31735062121391, Starzl CV No. 2229
Date Deposited: 08 Apr 2010 17:38
Last Modified: 12 Oct 2017 09:55
URI: http://d-scholarship.pitt.edu/id/eprint/5615

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item