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Active Immunosurveillance by CD8+ T Lymphocytes during Acute and Latent Herpes Simplex Virus-1 Infection

Khanna, Kamal Mohan (2004) Active Immunosurveillance by CD8+ T Lymphocytes during Acute and Latent Herpes Simplex Virus-1 Infection. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Herpes simplex virus type-1 (HSV-1) infection results in the establishment of a latent infection in sensory ganglia of the peripheral nervous system (PNS). In humans the virus can sporadically reactivate and gain access to the primary sites of infection and cause considerable tissue damage. The mechanisms involved in establishment and maintenance of latency and the sporadic reactivation of HSV-1 infected neurons in sensory ganglia is poorly defined. Elucidating the mechanisms involved in maintenance of latency of HSV-1 would be extremely valuable in controlling the high prevalence of disease in communities around the world. We have shown that CD8+ T lymphocytes can block HSV-1 reactivation from latency in sensory neurons of the trigeminal ganglion ex vivo. The mechanism that the CD8+ T cells employ to prevent reactivation includes release of the antiviral cytokine IFNã, but other mechanisms, such as the lytic effect of perforin and granzymes appear to be important as well. In order to further understand the cellular immune response against the latent HSV infection we have attempted to dissect the CD8+ T cell immune response in the TG during acute and latent infection. Specifically, we determined the antigen specificity and TCR expression of the CD8+ T cells that accumulate in the TG at various times post infection. We have investigated the state of CD8+ T cell memory after HSV-1 infection and clarified the mechanisms used by CD8+ T cells to prevent reactivation from latency.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Khanna, Kamal Mohankmkst65@pitt.eduKMKST65
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairHendricks, Robert L.
Committee MemberFlynn, JoAnne
Committee MemberFinn, Olivera J.
Committee MemberKinchington, Paul R.
Committee MemberReinhart, Todd A.
Date: 25 February 2004
Date Type: Completion
Defense Date: 17 February 2004
Approval Date: 25 February 2004
Submission Date: 25 February 2004
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Immunology
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: CD8 T cells; Herpes Simplex Virus; memory T cells; viral immunology
Other ID:, etd-02252004-135649
Date Deposited: 10 Nov 2011 19:31
Last Modified: 15 Nov 2016 13:36


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