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Jiang, Canping (2006) IMMOBILIZED COBALT AFFINITY PURIFICATION FOR HSV-1 BASED GENE THERAPY VECTORS. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Herpes simplex virus type 1 (HSV-1) is a promising vector for gene therapy applications. To be used as therapeutic agents, HSV-1 vectors must meet the stringent criteria of high titer and purity. Thu, development of scalable, efficient HSV-1 vector purification strategies is essential to advance HSV-1 vectors into clinic.In this dissertation, a novel, efficient HSV-1 vector purification method, based on immobilized metal affinity chromatography (IMAC), was developed. I first evaluated the feasibility of using various transition metal ions (Cu2+, Zn2+, Ni2+, and Co2+) for the purification of HSV-1 vectors. Results show that none of the metals investigated provided a means of separating the virus from impurities. However, of interest is the finding that neither the virus nor the impurities bound to immobilized Co2+, suggesting that this metal could be useful for HSV-1 vector purification if the vector could be endowed with the affinity toward cobalt. Accordingly, I constructed an HSV-1 recombinant bearing a cobalt affinity tag (HAT) in the heparan sulfate binding domain of the virion envelope glycoprotein B (gB). It was found that the productivity and infectivity of the tagged HSV-1 mutant (KgBHAT) was not adversely affected by the mutation; while the binding and elution of KgBHAT on cobalt charged iminodiacetate (IDA-Co2+) columns confirmed that efficient purification was possible. By reducing cobalt ion leakage and optimizing the loading conditions, flow rate, and chromatographic substrate, efficient purification of KgBHAT from crude supernatant was achieved with over 70% virus infectivity recovery and over 95% reduction in protein and DNA impurities.Finally, I found that purification of KgBHAT on IDA-Co2+ columns using crude supernatant as starting material resulted in significant loss in virus infectivity. Electron spinning resonance revealed that the virus inactivation was caused by hydroxyl free radicals generated from the interaction between cobalt ions and components in crude virus supernatant. Appropriate amounts of free radical scavenger, a free radical scavenger, or imidazole in the loading material was able to protect HSV-1 from inactivation, and led to high virus infectivity recovery from IMAC purification. This finding is the first report of free radical mediated biological inactivation in an actual IMAC purification.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAtaai, Mohammad
Committee MemberGlorioso, Joseph C.
Committee MemberKoepsel, Richard
Committee MemberFederspiel, William
Date: 2 June 2006
Date Type: Completion
Defense Date: 28 February 2006
Approval Date: 2 June 2006
Submission Date: 1 March 2006
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Chemical Engineering
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Ascorbate; Cobalt; Free Radical; Gene Therapy Vector; HSV-1; IMAC; Purification; Tag
Other ID:, etd-03012006-140224
Date Deposited: 10 Nov 2011 19:31
Last Modified: 15 Nov 2016 13:36


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