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Mineralized Tissue Engineering, Stem Cell Therapies and Proteomics Approaches

Teng, Pang-ning (2009) Mineralized Tissue Engineering, Stem Cell Therapies and Proteomics Approaches. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Cellular therapy holds tremendous potential in regeneration of mineralized tissues such as bones and teeth. We have characterized and identified pericytes as a unique population of dental pulp stem cells (DPSCs) that can be sorted by CD146+CD34-CD45-CD56-, expanded in culture, and differentiated into osteogenic, chondrogenic, and adipogenic lineages. A well-characterized stem cell source and an appropriate microenvironment containing growth factors and/or extracellular matrix (ECM) proteins to stimulate differentiation and mineralization are required for successful cellular therapies. To understand cell-ECM protein interaction, we studied the signaling role of phosphophoryn (PP), an ECM protein found in dentin and bone. PP signals through integrins, mitogen activated protein kinase (MAPK), and Smad pathways. There is also signaling crosstalk between the MAPK and Smad pathways. To better understand the complex signaling pathways involved in stem cell differentiation during dentin or bone formation, we have utilized quantitative proteomic strategies to study stem cell differentiation triggered by PP and BMP-2. Proteins upregulated and downregulated during differentiation were identified by mass spectrometry. With the ultimate goal of better enabling the regeneration of diseased or damaged mineralized tissue, our findings in this study have enhanced our understanding in stem cell differentiation to the osteoblastic/odontoblastic lineages and lay foundations for the development of future craniofacial regeneration.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSfeir, Charlescsfeir@pitt.eduCSFEIR
Committee MemberDay, Billybday@pitt.eduBDAY
Committee MemberPeault,
Committee MemberBorovetz, Harveyborovetzhs@upmc.eduBOROVETZ
Committee MemberWang, Tianyitiwang@pitt.eduTIWANG
Date: 29 June 2009
Date Type: Completion
Defense Date: 26 March 2009
Approval Date: 29 June 2009
Submission Date: 16 March 2009
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Proteomics; Stem Cells; Tissue Engineering
Other ID:, etd-03162009-115252
Date Deposited: 10 Nov 2011 19:32
Last Modified: 19 Dec 2016 14:35


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