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Cortical Layer-Dependent Hemodynamic Regulation Investigated by Functional Magnetic Resonance Imaging

Yen, Cecil Chern-Chyi (2011) Cortical Layer-Dependent Hemodynamic Regulation Investigated by Functional Magnetic Resonance Imaging. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Functional magnetic resonance imaging (fMRI) is currently one of the most widely used non-invasive neuroimaging modalities for mapping brain activation. Techniques such as blood oxygenation level dependent (BOLD) fMRI or cerebral blood volume (CBV)-weighted fMRI are based on the assumption that hemodynamic responses are tightly regulated by neural activity. However, the relationship between fMRI responses and neural activity is still unclear. To investigate this relationship, the unique properties of temporal frequency tuning of primary visual cortex neurons was used as a model since it can be used to separate the neural input and output activities of this area. During moving grating stimuli of 1, 2, 10 and 20 Hz temporal frequencies, two fMRI studies, areal and laminar studies, were conducted with different spatial resolution in a 9.4-T Varian spectrometer. In areal studies, BOLD fMRI was able to detect the difference in tuning properties between area 17 (A17), area 18 (A18) and lateral geniculate nucleus. In A17, the BOLD tuning curve seemed to reflect the local field potential (LFP) low frequency band (<12 Hz) rather than spiking activity and LFP gamma band (25-90 Hz). In laminar studies, a high spatial resolution protocol was adopted to resolve the different cortical layers in A17. In addition to BOLD fMRI, CBV-weighted fMRI was performed to eliminate the contamination from the superficial draining veins. These results showed that BOLD and CBV tuning curves do not reflect the underlying spiking activity or the LFP activity at infragranular layers (the bottom layer of three cortical layers). This implies that the hemodynamic response may not be regulated on a laminar level. Therefore, caution should be taken when interpreting BOLD responses as the sole indicator of different aspects of neural activity in areal and laminar scales.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Yen, Cecil Chern-Chyichy25@pitt.eduCHY25
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairKim, Seong-Gikimsg@pitt.eduKIMSG
Committee MemberStetten, George
Committee MemberAizenstein, Howard J.aizen@pitt.eduAIZEN
Committee MemberCrowley, Justin
Date: 19 September 2011
Date Type: Completion
Defense Date: 13 April 2011
Approval Date: 19 September 2011
Submission Date: 23 March 2011
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: CBV; Layer; fMRI; Hemodynamic; MRI; BOLD
Other ID:, etd-03232011-173811
Date Deposited: 10 Nov 2011 19:32
Last Modified: 15 Nov 2016 13:37


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