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Investigation of Genetic Association of MMP10 with Dental Caries

Commander, Sara B (2011) Investigation of Genetic Association of MMP10 with Dental Caries. Master's Thesis, University of Pittsburgh. (Unpublished)

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Dental caries is the most prevalent chronic disease seen in children worldwide, and despite advancements in oral healthcare, many adults and children are still affected. The etiology of dental caries is complex, including environmental and genetic factors. Environmental factors such as fluoride exposure, oral hygiene, salivary function, diet, and bacterial flora have all been studied and found to contribute to dental caries experience. However, little is known about the genetic causes of dental caries. Candidate genes involved in enamel formation, tooth development, and taste have all been studied and found to be associated with dental caries.Matrix metalloproteinases are a multi-gene family responsible for degrading extracellular matrix (ECM) molecules. Their role in the oral environment involves ECM remodeling and degradation processes. Because the dentin of a tooth is composed primarily of an organic matrix, mostly collagen, it can pose as a potential substrate for MMP10.Recent initial GWAS results showed an association between MMP10 and dental caries [Personal Communication, Center for Craniofacial and Dental Genetics].Based upon these initial GWAS findings, we performed a replication study to determine if the associations between MMP10 and dental caries seen in the initial GWAS were present in our population. We also studied genotype precision between our study and that of the initial GWAS. We genotyped 274 Caucasian individuals at SNP rs2276108 and 338 Caucasian individuals at SNP rs17293642 using a Taqman® SNP genotyping assay. Linear regression analysis using PLINK, adjusting for age, sex and site, found no association between the SNPs and dental caries in our sample population. Failure to replicate the initial GWAS findings in MMP10 suggests priority efforts should be given to exploring other candidate associations. The public health importance of identifying these genetic factors and understanding how they impact dental caries and other oral heatlh issues should open up new strategies for treatment and prevention and may elucidate new biological processes.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Commander, Sara
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairWendell, Stevenwend0017@pitt.eduWEND0017
Committee MemberFinegold, Daviddnf@pitt.eduDNF
Committee MemberGrubs, Robinrgrubs@pitt.eduRGRUBS
Date: 29 June 2011
Date Type: Completion
Defense Date: 6 April 2011
Approval Date: 29 June 2011
Submission Date: 4 April 2011
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Genetic Counseling
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: dental caries; GWAS; MMP10; SNPs
Other ID:, etd-04042011-205707
Date Deposited: 10 Nov 2011 19:34
Last Modified: 15 Nov 2016 13:38


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