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The Effect of Epigallocatechin gallate (EGCG) on Mycobacterium tuberculosis and Mycobacterium smegmatis

Seldina, Yuliya (2011) The Effect of Epigallocatechin gallate (EGCG) on Mycobacterium tuberculosis and Mycobacterium smegmatis. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Objectives/Research questions: Epigallocatechin gallate (EGCG) is a major catechin (antioxidant) component of green tea. Recent studies have shown that it possesses many health benefits, including antimicrobial activity against some bacteria and viruses. Due to its known antimicrobial activity, this study focused on exploring the effects of EGCG on the growth of Mycobacterium tuberculosis, the causative agent of tuberculosis, as it remains a major public health concern. Through the use of a model mycobacterial species, Mycobacterium smegmatis, the experiments investigated the effects of varying concentrations of EGCG on mycobacteria.Methods: The effects of EGCG on the growth of M. tuberculosis and M. smegmatis were explored by setting up serial dilutions and carrying out cell counts over certain time periods. High Pressure Liquid Chromatography (HPLC) was used to fractionate the EGCG in order to better understand its behavior in the media. Results: The study has found that medium containing EGCG has anti-mycobacterial activity, but it is not EGCG itself that carries it out but a degradant molecule. This observation was achieved following experiments showing that a 3-day pre-incubation of EGCG in the medium strengthens its anti-mycobacterial activity. However, the HPLC showed that EGCG is fully degraded by day 1. Therefore, by day 3, there are only degradant molecules in the medium. Findings were consistent with an experiment which found that a pre-incubated green tea extract has more activity against the cells than a fresh one. Conclusions: A three day pre-incubation of green tea extract at 37°C enhances anti-mycobacterial activity, which may have implications in using green tea as a prophylactic agent. Implications for public health: If EGCG has antimicrobial activity green tea could be used as a prophylactic agent against Mycobacterium tuberculosis.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Seldina, Yuliyayuliya.seldina@gmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairOjha, Anilano7@pitt.eduANO7
Committee MemberLombardi, Emiliaell8@pitt.eduELL8
Committee MemberRohan, Lisalrohan@mwri.magee.edu
Committee MemberReinhart, Toddreinhar@pitt.eduREINHAR
Date: 29 June 2011
Date Type: Completion
Defense Date: 4 April 2011
Approval Date: 29 June 2011
Submission Date: 5 April 2011
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MPH - Master of Public Health
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: antimycobacterial; tuberculosis; EGCG; green tea
Other ID: http://etd.library.pitt.edu/ETD/available/etd-04052011-185907/, etd-04052011-185907
Date Deposited: 10 Nov 2011 19:34
Last Modified: 15 Nov 2016 13:38
URI: http://d-scholarship.pitt.edu/id/eprint/6800

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