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Sero-Epidemiological Studies on Human Herpes Virus-8

Hoffman, Linda J. (2005) Sero-Epidemiological Studies on Human Herpes Virus-8. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Abstract

Human herpes virus-8 (HHV-8) is a known carcinogenic agent. This report investigates five populations to determine if HHV-8 was associated with disease onset. Detection and levels of antibodies were measured using an enhanced immunofluorescent assay and were analyzed with the statistical program, SPSS.In the first study, we tested the hypothesis that HHV-8 was the causative agent in Langerhan's cell histiocytosis (LCH). The seroprevalence of HHV-8 among 159 LCH patients was similar to the control group, indicating that HHV-8 is not the etiological agent of LCH.In the second study, we tested the hypothesis that HHV-8 reactivation occurs in solid-organ transplant (SOT) patients, following immunosuppression. We found a significant increase in HHV-8 seropositivity when comparing pre-transplant to post-transplant samples (p-less than .01). There was also an overall increase in viral antibody titers following transplantation (p=less than .001), indicating viral reactivation. In the third study, we compare the SOT results to bone-marrow transplant patients (BMT). Longitudinal serum samples from 34 BMT patients did not demonstrate a significant association with HHV-8 as compared to the control (p=.716) or the SOT populations (p=.180). In addition, HHV-8 reactivation did not occur post-transplantation.In the fourth study, we tested the hypothesis that HHV-8 is associated with increased risk of prostate cancer (PrCa). There was greater than a 2-fold association between HHV-8 seroprevalence and PrCa among African-Caribbean men from Tobago (p=.003). A similar trend was present in a PrCa cohort from the United States, p=greater than .05.In a fifth study, we tested the hypothesis that HHV-8 increased the risk of PrCa among men who carried genetic polymorphisms in the androgen (AR) and estrogen receptor (ESR1) genes. This study analyzed an expanded Tobago cohort, which demonstrated an association between HHV-8 and PrCa (OR 1.74, p=.032). An increased association was found among seropositive men carrying the high-risk AR allele (OR=2.46, p=.023) and ESR1 allele (OR=3.10, p=.004). The strongest association was found in seropositive men with both high-risk alleles (OR=5.20, p=.017).This study demonstrates the use of HHV-8 serology as a marker for an increased public health cancer detectable risk, due to viral prevalence or reactivation.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hoffman, Linda J.hoffmanlj@comcast.net
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairJenkins, Frank JFjJenkins@pitt.edu
Committee MemberRinaldo, Charles RRinaldo@pitt.eduRINALDO
Committee MemberMilcarek, ChristineMilcarek@pitt.eduMILCAREK
Committee MemberBunker, Clareann HBunkerC@pitt.eduBUNKERC
Date: 9 June 2005
Date Type: Completion
Defense Date: 20 December 2004
Approval Date: 9 June 2005
Submission Date: 13 April 2005
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: cancer; carcinogenic; herpesvirus; Human herpesvirus-8; KSHV; epidemiology; HHV-8; public health
Other ID: http://etd.library.pitt.edu/ETD/available/etd-04132005-195044/, etd-04132005-195044
Date Deposited: 10 Nov 2011 19:36
Last Modified: 15 Nov 2016 13:40
URI: http://d-scholarship.pitt.edu/id/eprint/7095

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