Topper, Sarah L. (2011) Extra-column Band Broadening in Ultra High Performance Liquid Chromatography. Master's Thesis, University of Pittsburgh.
Abstract
Advances in column technologies for high performance liquid chromatography (HPLC) have led to the use of small, highly efficient packing materials. The use of these materials requires short, small diameter columns as well as instruments capable of withstanding high pressures (up to 1000bar) and sometimes temperature (in excess of 100°C), a technique dubbed ultra-high performance liquid chromatography or UPLC. The advantage is a greater than ten-fold reduction in analysis time without a loss of peak capacity or resolution. Due to the small volumes inherent in the new columns, the extra-column volumes of the instrument can become a significant source of dispersion leading to extra-column broadening of chromatographic peaks. Uncontrolled or accounted for, this variance severely limits the separation potential of improved column packings and reduces the accuracy of evaluations of instruments and columns. An investigation is made of the source and nature of the band broadening in instrumental components with an eye towards reduction of variance without loss of performance. Different methods for calculating the degree of extra-column band broadening are discussed. Applications of the calculated data for evaluation of the kinetic parameters of UPLC are reviewed.
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Details |
| Item Type: | University of Pittsburgh ETD |
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| ETD Committee: | | ETD Committee Type | Committee Member | Email |
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| Committee Chair | Weber, Stephen G | sweber@pitt.edu | | Committee Member | Michael, Adrian C | amichael@pitt.edu | | Committee Member | Trakselis, Michael A | mtraksel@pitt.edu |
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| Title: | Extra-column Band Broadening in Ultra High Performance Liquid Chromatography |
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| Status: | Unpublished |
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| Abstract: | Advances in column technologies for high performance liquid chromatography (HPLC) have led to the use of small, highly efficient packing materials. The use of these materials requires short, small diameter columns as well as instruments capable of withstanding high pressures (up to 1000bar) and sometimes temperature (in excess of 100°C), a technique dubbed ultra-high performance liquid chromatography or UPLC. The advantage is a greater than ten-fold reduction in analysis time without a loss of peak capacity or resolution. Due to the small volumes inherent in the new columns, the extra-column volumes of the instrument can become a significant source of dispersion leading to extra-column broadening of chromatographic peaks. Uncontrolled or accounted for, this variance severely limits the separation potential of improved column packings and reduces the accuracy of evaluations of instruments and columns. An investigation is made of the source and nature of the band broadening in instrumental components with an eye towards reduction of variance without loss of performance. Different methods for calculating the degree of extra-column band broadening are discussed. Applications of the calculated data for evaluation of the kinetic parameters of UPLC are reviewed. |
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| Date: | 06 June 2011 |
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| Date Type: | Completion |
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| Defense Date: | 19 April 2011 |
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| Approval Date: | 06 June 2011 |
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| Submission Date: | 19 April 2011 |
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| Access Restriction: | No restriction; Release the ETD for access worldwide immediately. |
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| Patent pending: | No |
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| Institution: | University of Pittsburgh |
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| Thesis Type: | Master's Thesis |
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| Refereed: | Yes |
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| Degree: | MS - Master of Science |
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| URN: | etd-04192011-120702 |
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| Uncontrolled Keywords: | capillary; Gaussian; injector; variance |
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| Schools and Programs: | Dietrich School of Arts and Sciences > Chemistry |
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| Date Deposited: | 10 Nov 2011 14:39 |
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| Last Modified: | 23 May 2012 13:28 |
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| Other ID: | http://etd.library.pitt.edu/ETD/available/etd-04192011-120702/, etd-04192011-120702 |
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