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Extra-column Band Broadening in Ultra High Performance Liquid Chromatography

Topper, Sarah L. (2011) Extra-column Band Broadening in Ultra High Performance Liquid Chromatography. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Advances in column technologies for high performance liquid chromatography (HPLC) have led to the use of small, highly efficient packing materials. The use of these materials requires short, small diameter columns as well as instruments capable of withstanding high pressures (up to 1000bar) and sometimes temperature (in excess of 100°C), a technique dubbed ultra-high performance liquid chromatography or UPLC. The advantage is a greater than ten-fold reduction in analysis time without a loss of peak capacity or resolution. Due to the small volumes inherent in the new columns, the extra-column volumes of the instrument can become a significant source of dispersion leading to extra-column broadening of chromatographic peaks. Uncontrolled or accounted for, this variance severely limits the separation potential of improved column packings and reduces the accuracy of evaluations of instruments and columns. An investigation is made of the source and nature of the band broadening in instrumental components with an eye towards reduction of variance without loss of performance. Different methods for calculating the degree of extra-column band broadening are discussed. Applications of the calculated data for evaluation of the kinetic parameters of UPLC are reviewed.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Topper, Sarah L.stopper@ppg.comstopper@ppg.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairWeber, Stephen Gsweber@pitt.edusweber@pitt.edu
Committee MemberMichael, Adrian Camichael@pitt.eduamichael@pitt.edu
Committee MemberTrakselis, Michael Amtraksel@pitt.edumtraksel@pitt.edu
Date: 6 June 2011
Date Type: Completion
Defense Date: 19 April 2011
Approval Date: 6 June 2011
Submission Date: 19 April 2011
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: capillary; Gaussian; injector; variance
Other ID: http://etd.library.pitt.edu/ETD/available/etd-04192011-120702/, etd-04192011-120702
Date Deposited: 10 Nov 2011 19:39
Last Modified: 23 May 2012 17:28
URI: http://d-scholarship.pitt.edu/id/eprint/7338

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