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Tataurov, Andrey V (2005) MEASUREMENT OF LATERAL DIFFUSION IN LIPIDS. Master's Thesis, University of Pittsburgh. (Unpublished)

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Different techniques to study lateral diffusion of lipids are reviewed, including long-range diffusion methods and short-range diffusion methods. Long-range diffusion methods require the measurements of the time needed to fill a given area of the membrane. Applications of fluorescence recovery after photobleaching (FRAP) method are discussed. Short-range diffusion methods measure the rate of bimolecular collisions. The methods include fluorescence quenching, fluorescence energy transfer, nuclear magnetic resonance and electron spin resonance spectroscopy. Special attention is given to Electron Spin Resonance (ESR) spectroscopy. Procedure of obtaining diffusion coefficient D using novel two dimensional electron double-double resonance experiment (2D-ELDOR) is discussed in greater detail. In order to demonstrate the feasibility of using 2D Fourier Transform ESR spectroscopy for lateral diffusion studies, the D of spin-labeled phospholipids sample is estimated by 2D-ELDOR method.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Tataurov, Andrey Vavt1@pitt.eduAVT1
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSaxena, Sunilsksaxena@pitt.eduSKSAXENA
Committee MemberAmemiya, Shigeruamemiya@pitt.eduAMEMIYA
Committee MemberPetoud, Stephanespetoud@pitt.eduSPETOUD
Date: 9 June 2005
Date Type: Completion
Defense Date: 19 April 2005
Approval Date: 9 June 2005
Submission Date: 20 April 2005
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: 2D-ELDOR; diffusion coefficient; EPR; ESR; lateral diffusion; lipids
Other ID:, etd-04202005-224253
Date Deposited: 10 Nov 2011 19:39
Last Modified: 15 Nov 2016 13:41


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