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VASP and Profilin-1 Interaction in Cell Migration

Gau, David Martin (2011) VASP and Profilin-1 Interaction in Cell Migration. Undergraduate Thesis, University of Pittsburgh. (Unpublished)

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Profilins belong to a family of G-actin binding proteins which are thought to facilitate actinpolymerization at the leading edge of migrating cells via its polyproline interactions with majoractin nucleating and F-actin elongating proteins. The two major goals of this study are i) tospatially resolve profilins-1's (the only ubiquitously expressed member of profilin family)interaction with Ena (enabled)/VASP (vasodilator stimulated phosphoprotein) family of F-actinelongating protein, and ii) determine whether Ena/VASP regulates cell migration through itsinteraction with profilin-1. This study demonstrates the feasibility of GFP (green fluorescence protein)-based florescence resonance energy transfer (FRET) to identify profilin-1 and VASP interaction. Through acceptor photobleaching FRET (fluorescence resonance energy transfer) inMDA-MB-231 human breast cancer cells, we show that VASP and profilin-1 interaction at themembrane ruffles near the leading edge. We further show that VASP overexpression in breastcancer cells results in slower random cell motility; however VASP-induced suppression of cellmotility is partly rescued when VASP:profilin-1 interaction is downregulated These data suggestVASP utilizes profilin-1 to regulate breast cancer cell migration.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Gau, David Martindmg40@pitt.eduDMG40
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee Chair Roy, Parthapar19@pitt.eduPAR19
Committee MemberBaty, Catherinecjb16@pitt.eduCJB16
Committee MemberGertler,
Committee MemberShroff, Sanjeevsshroff@pitt.eduSSHROFF
Date: 2 May 2011
Date Type: Completion
Defense Date: 15 April 2011
Approval Date: 2 May 2011
Submission Date: 20 April 2011
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
David C. Frederick Honors College
Degree: BS - Bachelor of Science
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: Cell Migration; Ena/VASP; FRET; MDA-MB-231; Profilin-1
Other ID:, etd-04202011-103456
Date Deposited: 10 Nov 2011 19:39
Last Modified: 15 Nov 2016 13:41


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