Gau, David Martin
(2011)
VASP and Profilin-1 Interaction in Cell Migration.
Undergraduate Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Profilins belong to a family of G-actin binding proteins which are thought to facilitate actinpolymerization at the leading edge of migrating cells via its polyproline interactions with majoractin nucleating and F-actin elongating proteins. The two major goals of this study are i) tospatially resolve profilins-1's (the only ubiquitously expressed member of profilin family)interaction with Ena (enabled)/VASP (vasodilator stimulated phosphoprotein) family of F-actinelongating protein, and ii) determine whether Ena/VASP regulates cell migration through itsinteraction with profilin-1. This study demonstrates the feasibility of GFP (green fluorescence protein)-based florescence resonance energy transfer (FRET) to identify profilin-1 and VASP interaction. Through acceptor photobleaching FRET (fluorescence resonance energy transfer) inMDA-MB-231 human breast cancer cells, we show that VASP and profilin-1 interaction at themembrane ruffles near the leading edge. We further show that VASP overexpression in breastcancer cells results in slower random cell motility; however VASP-induced suppression of cellmotility is partly rescued when VASP:profilin-1 interaction is downregulated These data suggestVASP utilizes profilin-1 to regulate breast cancer cell migration.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
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| ETD Committee: |
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| Date: |
2 May 2011 |
| Date Type: |
Completion |
| Defense Date: |
15 April 2011 |
| Approval Date: |
2 May 2011 |
| Submission Date: |
20 April 2011 |
| Access Restriction: |
5 year -- Restrict access to University of Pittsburgh for a period of 5 years. |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Swanson School of Engineering > Bioengineering
David C. Frederick Honors College |
| Degree: |
BS - Bachelor of Science |
| Thesis Type: |
Undergraduate Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Cell Migration; Ena/VASP; FRET; MDA-MB-231; Profilin-1 |
| Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-04202011-103456/, etd-04202011-103456 |
| Date Deposited: |
10 Nov 2011 19:39 |
| Last Modified: |
15 Nov 2016 13:41 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/7389 |
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