Belasco, Laura
(2007)
The Development and Characterization of 'Rod-Hinge-Rod'-type Molecular Actuators.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
We have successfully synthesized a bivalent hinged molecule that binds metal to undergolarge conformational changes, similar to an actuator. Spectrophotometric titrations aswell as a Job plot have shown that binding occurs on a 1:1 ratio of metal to hinged molecule. Addition of EDTA as a metal scavenger causes the molecule to return to the unbound state. Gel filtration chromatography has shown the effects of metal binding on the overall size and shape of the actuator molecule; the "closed" form is much smaller than the "open" form, indicating that large conformational changes are occurring upon binding. Analytical ultracentrifugation has unambiguously confirmed that the molecules are binding metal in a monomeric fashion.
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| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
13 June 2007 |
| Date Type: |
Completion |
| Defense Date: |
5 April 2007 |
| Approval Date: |
13 June 2007 |
| Submission Date: |
25 April 2007 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
bis-amino acid; macromolecules; molecular scaffolds; nanoscience |
| Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-04252007-112901/, etd-04252007-112901 |
| Date Deposited: |
10 Nov 2011 19:42 |
| Last Modified: |
15 Nov 2016 13:42 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/7637 |
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