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The Development and Characterization of 'Rod-Hinge-Rod'-type Molecular Actuators

Belasco, Laura (2007) The Development and Characterization of 'Rod-Hinge-Rod'-type Molecular Actuators. Master's Thesis, University of Pittsburgh. (Unpublished)

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We have successfully synthesized a bivalent hinged molecule that binds metal to undergolarge conformational changes, similar to an actuator. Spectrophotometric titrations aswell as a Job plot have shown that binding occurs on a 1:1 ratio of metal to hinged molecule. Addition of EDTA as a metal scavenger causes the molecule to return to the unbound state. Gel filtration chromatography has shown the effects of metal binding on the overall size and shape of the actuator molecule; the "closed" form is much smaller than the "open" form, indicating that large conformational changes are occurring upon binding. Analytical ultracentrifugation has unambiguously confirmed that the molecules are binding metal in a monomeric fashion.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Belasco, Lauralgb4@pitt.eduLGB4
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSchafmeister, Christianmeister@pitt.eduMEISTER
Committee MemberKoide, Kazunorikoide@pitt.eduKOIDE
Committee MemberMeyer, Taratmeyer@pitt.eduTMEYER
Date: 13 June 2007
Date Type: Completion
Defense Date: 5 April 2007
Approval Date: 13 June 2007
Submission Date: 25 April 2007
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: bis-amino acid; macromolecules; molecular scaffolds; nanoscience
Other ID:, etd-04252007-112901
Date Deposited: 10 Nov 2011 19:42
Last Modified: 15 Nov 2016 13:42


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