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Moldovan, Radu Gheorghe (2006) THE INTERACTION BETWEEN LAMBDA PHAGE AND ITS BACTERIAL HOST. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Our interest in the adsorption of lambda phages onto bacterial cells was triggered by the controversial results Schwartz obtained in the 70's (Schwartz 1976). Lambda phages bind to specific receptors, named LamB, on the cell's surface during the infection process. Phage adsorption onto the cell wall is a diffusion-limited process. One of the controversies is the rate of adsorption, which in some cases appears to exceed the theoretical limit imposed by the physical law of random diffusion. We revisited this problem by carrying out experiments along with new theoretical analyses. Our measurements show that the population of unbound phages decreases with time in a double-exponential fashion. Using fluorescence microscopy we quantified the number of receptors per cell. This dissertation describes the adsorption of lambda phages onto their host cells and a kinetics model, which allows the calculation of adsorption, desorption, and irreversible binding rates from a single measurement. The long-term interaction between lambda phage and its bacterial host in a co-habitual environment is approached as well. A complex mathematical model describing the dynamics of the two populations (bacteria and phages) is presented along with experimental results. Surprising phenomena of bacterial persistence against phage infection are also reported.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Moldovan, Radu
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairWu, Xiao-lunxlwu@pitt.eduXLWU
Committee MemberJasnow, Davidjasnow@pitt.eduJASNOW
Committee MemberCoalson, Robertrob@ringo.chem.pitt.eduCOALSON
Committee MemberDuda, Robertduda@pitt.eduDUDA
Committee MemberHendrix, Rogerrhx@pitt.eduRHX
Date: 2 June 2006
Date Type: Completion
Defense Date: 22 November 2005
Approval Date: 2 June 2006
Submission Date: 27 April 2006
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Physics
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: E. coli; fluorescence; kinetics; lambda; population dynamics
Other ID:, etd-04272006-134555
Date Deposited: 10 Nov 2011 19:42
Last Modified: 15 Nov 2016 13:42


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