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The Denaturation of Cytochrome c and Cytochrome c as Peroxidase

Wang, Lei (2006) The Denaturation of Cytochrome c and Cytochrome c as Peroxidase. Master's Thesis, University of Pittsburgh. (Unpublished)

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Conformational transitions of proteins play a crucial role in many biochemical and biophysical reactions. Understanding the conformational changes of a protein upon adsorption to a substrate is very important in biotechnology, e.g. the development of modern protein chip technology, biocompatibility of implants, and many other ones. This research program used cytochrome c, an electron carrier in the respiratory chain, as a model to probe how surface adsorption affects the folding of a protein. My work investigates the interaction of protein horse heart cytochrome c when it is electrostatically adsorbed or covalently attached onto a 1 nm thick monolayer film, which covers an Au surface. After changing the pH value of the solutions or adding the denaturants into the solutions, the conformation of cytochrome c changes and causes a change of the peak current of cyclic voltammgram. In addition, the denatured cytochrome c¡¯s peroxidase activity will be studied and compared with the peroxidase activity of microperoxidase-11.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Wang, Leilew16@pitt.eduLEW16
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairWaldeck, David Hdave@pitt.eduDAVE
Committee MemberMichael, Adrian Camichael@pitt.eduAMICHAEL
Committee MemberSaxena, Sunil Ksksaxena@pitt.eduSKSAXENA
Date: 28 September 2006
Date Type: Completion
Defense Date: 26 January 2006
Approval Date: 28 September 2006
Submission Date: 27 April 2006
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: cyclic voltammogram; cytochrome c; gold ball electode; hydrogen peroxide
Other ID:, etd-04272006-170820
Date Deposited: 10 Nov 2011 19:42
Last Modified: 15 Nov 2016 13:42


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