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The Characterization of Drosophila Shroom

Zasadil, Lauren Marie (2010) The Characterization of Drosophila Shroom. Undergraduate Thesis, University of Pittsburgh. (Unpublished)

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Many complex mechanisms regulate cytoskeletal-dependant changes in cell morphology and behavior embryonic and adult life. The actin-binding protein Shroom 3 (Shrm3) appears to interact with Rho kinase (Rok) to direct the assembly of a contractile actomyosin network in neuroepithelial cells, causing apical constriction during neural tube formation. An ortholog of Shrm3 was identified, Drosophila Shroom (dShrm), that contains homology to the Shrm3 domain responsible for constriction. When properly targeted, the invertebrate domain also exhibits the ability to cause apical constriction, suggesting that the pathway is conserved in invertebrates. Drosophila melanogaster will likely provide a powerful model system to study the localization and function of Shrm during tissue morphogenesis. The project has three goals: to classify the endogenous expression of dShrm, to investigate the effects of over expressing dShrm in various tissues, and to determine a potential interaction between dShrm and dRok. There are two isoforms of dShrm we have primarily studied, dShrmA and dShrmB. Using in situ hybridization and immunohistochemistry, we have shown that endogenous dShrm proteins localizes to adherens junctions (dShrmA) and the apical plasma membranes (dShrmB) of cells in the ectoderm, trachea system, and other cell populations during embryogenesis. Over expression of dShrm in the ectoderm, as well as the eye and wing imaginal discs, causes dramatic defects in tissue architecture. Over expression of dShrmA with dRok has shown that there is an interaction between these two proteins. Further work will focus on defining the mechanism by which dShrm functions and which tissues require its activity during embyogenesis.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Zasadil, Lauren
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairHildebrand, Jeffreyjeffh@pitt.eduJEFFH
Committee MemberStronach, Bethstronach@pitt.eduSTRONACH
Committee MemberMcCartney,
Committee MemberTwombly, Vernontwombly@pitt.eduTWOMBLY
Date: 14 May 2010
Date Type: Completion
Defense Date: 22 April 2010
Approval Date: 14 May 2010
Submission Date: 4 May 2010
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
David C. Frederick Honors College
Degree: BPhil - Bachelor of Philosophy
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: apical constriction; development; Drosophila; dShrm; Rok; Shrm; Shroom
Other ID:, etd-05042010-145857
Date Deposited: 10 Nov 2011 19:43
Last Modified: 15 Nov 2016 13:43


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