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Following Dynamics of Protein/Nucleic Acid Interactions in Real-Time

Kim, Min Sun (2008) Following Dynamics of Protein/Nucleic Acid Interactions in Real-Time. Master's Thesis, University of Pittsburgh. (Unpublished)

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Methyl-CpG binding protein 2 (MeCP2) is a protein associated with transcriptional repression of other proteins and its various mutants are found in Rett syndrome patients, which is a severe neurodevelopmental disease found in 1/15,000 females. It has specific binding affinity to methyl-CpG domain but also bind to architectural DNA structure. Here, its binding dynamics to Holliday junction structure was investigated by using single molecular detections; scanning confocal fluorescence microscope and wide-field evanescent field fluorescence microscope. Through these studies, we could distinguish the difference of transitions of the substrate with and without MeCP2. The effect of this MeCP2 binding to 4WJ will be discussed in terms of the structures and transition times.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Kim, Min
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairLeuba, Sanford Hleuba@pitt.eduLEUBA
Committee MemberKlein-Seetharaman,
Committee MemberSteinman, Richard Asteinman@pitt.eduSTEINMAN
Committee MemberWetzel, Ronard Brwetzel@pitt.eduRWETZEL
Committee MemberKhan, Saleem Akhan@pitt.eduKHAN
Date: 25 September 2008
Date Type: Completion
Defense Date: 21 November 2007
Approval Date: 25 September 2008
Submission Date: 8 May 2008
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Molecular Biophysics
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: 4WJ; EFFM; MeCP2; SCFM; spFRET
Other ID:, etd-05082008-142324
Date Deposited: 10 Nov 2011 19:44
Last Modified: 15 Nov 2016 13:43


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