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The role of the Lim1 gene in vertebrate kidney development

Ye, Mao (2008) The role of the Lim1 gene in vertebrate kidney development. Master's Thesis, University of Pittsburgh. (Unpublished)

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Lim1 has been reported to regulate the head, nerve system and kidney development in mouse, zebrafish and Xenopus. In the present study, we demonstrate that Xlim1 plays a pivotal role in the pronephric specification and development in Xenopus.In Xenopus embryos at 4-cell and 8-cell stage, loss of function experiments using DEED depletion showed that down-regulation of Xlim1 expression severely impairs the formation of pronephros, assessed by pax8 expression. Overexpressing Xlim1 by injecting constitutively active Lim1 constructs in Xenopus embryos induce remarking enlargement of pronephros at various stages during development, assessed by both in situ hybridization and immunohistochemistry staining. Furthermore we studied the working window of Xlim1 on pronephric specification in mesoderm and observed the peak time point is at stage 10.5 while the whole working window is between stage 9 and 12.5. To understand the mechanism of Xlim1's regulation in kidney development, then we performed the microarray analysis on the induced pronephric RNA sample extracted from animal caps, targeting couple of genes that either are the members involved in the lim1 pathway or in the networking that induce the pronephric specification.In summary, our data suggest that Lim1 acts as a potent factor that determines the early specification of pronephros. Its expression levels at the early stage critically influences the specification of the pronephric tubules. More knowledge about XLim1's pathway in kidney development will be studied via examining the gene targets screened from microarray analysis.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSchimdt, Martinmcs2@pitt.eduMCS2
Committee MemberHukriede, Neilhukriede@pitt.eduHUKRIEDE
Committee MemberSmithgall, Thomastsmithga@pitt.eduTSMITHGA
Date: 30 June 2008
Date Type: Completion
Defense Date: 5 June 2008
Approval Date: 30 June 2008
Submission Date: 9 June 2008
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: pronephros Xenopus microarray Xlim1
Other ID:, etd-06092008-153129
Date Deposited: 10 Nov 2011 19:46
Last Modified: 15 Nov 2016 13:44


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