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Diffusion and Solid State NMR Studies of Structures in Model Biological Membranes

Cho, Hyo Soon (2011) Diffusion and Solid State NMR Studies of Structures in Model Biological Membranes. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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We studied the structures in model biological membranes using solid state NMR and NMR diffusion microscopy. We have created a stable bicelle system containing cholesterol, unsaturated and saturated lipids, capable of forming micron-scale lipid domains and suitable for structural biology studies of membrane proteins. The domains were observed by measuring time-dependent diffusion constants reflecting restricted diffusion of the lipids within micron-scale regions of the bicelles. We observed the correlation of bilayer structure of diverse bicelle systems with their alignment capability, using 31P NMR to measure the density of perforations in the bilayer as a function of bicelle alignment. We found a critical density for optimal bicelle alignment, applicable to bicelles of different lipid compositions. We were able to measure the effect of the perforation density on micron-scale domain formation in lipid bilayers. The perforations redistributed line active lipids within the bilayer, affecting the bulk line tension of the lipid domain. This work demonstrated that membrane topology can control domain formation.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Cho, Hyo
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSpence, Megan
Committee MemberWaldeck, David
Committee Membervan der Wel, Patrick
Committee MemberSaxena, Sunil
Date: 16 September 2011
Date Type: Completion
Defense Date: 23 June 2011
Approval Date: 16 September 2011
Submission Date: 28 June 2011
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: bicelle; diffusion
Other ID:, etd-06282011-163133
Date Deposited: 10 Nov 2011 19:48
Last Modified: 14 Mar 2023 20:20


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