Khanna, Raghav
(2011)
Pathogen Detection with Loop Mediated Isothermal Amplification.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Recently, a novel methodology termed Loop-mediated isothermal amplification (LAMP) was reported as the preferred technique for rapid diagnostic testing of pathogens. The advantages of LAMP over older techniques are its economic viability, rapidity and its obviation of complex instruments. Reported here is an interdisciplinary approach between medical practices and engineering to implement an affordable diagnostic device which employs LAMP for detection of pathogens. LAMP involves the optical excitation and detection of a pathogen sample mixed with a fluorescent dye as it is heated and amplified over an hour. The device reported here consists of readily available components which heat, optically excite and detect a LAMP sample. Finally, the integration will graphically display the amplification of the LAMP sample as a function of time on a palm top computer by exploiting the ubiquitous 802.11 wireless standard. The diagnostic box implemented here and its supporting components accurately discriminated between positive (infected) and negative (not infected) LAMP samples of various pathogens in approximately one hour. These results were verified using the standard method of pathogen diagnosis termed electrophoresis. Due to the low cost and portability of the device reported here, it poses as a potential solution to the need for quality point of care diagnostic tools in developing countries.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
3 August 2011 |
| Date Type: |
Completion |
| Defense Date: |
14 July 2010 |
| Approval Date: |
3 August 2011 |
| Submission Date: |
20 July 2010 |
| Access Restriction: |
5 year -- Restrict access to University of Pittsburgh for a period of 5 years. |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Swanson School of Engineering > Electrical Engineering |
| Degree: |
MSEE - Master of Science in Electrical Engineering |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Bio-instrumentation; LAMP; optics; PCR |
| Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-07202010-130229/, etd-07202010-130229 |
| Date Deposited: |
10 Nov 2011 19:52 |
| Last Modified: |
15 Nov 2016 13:46 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/8471 |
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