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The Effect of Extracellular Cardiolipin in Bovine Pulmonary Artery Endothelial Cells

Liu, Shannen Yuan-Chun (2010) The Effect of Extracellular Cardiolipin in Bovine Pulmonary Artery Endothelial Cells. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Group B Streptococcus is the most common cause of bacterial infection in the newborns. Infection by Group B Streptococcus often results in pulmonary hypertension. The bacterial components causing pulmonary hypertension had been identified as cardiolipin and phosphatidylglycerol. Cardiolipin not only induces pulmonary hypertension in human, but also in lambs. Due to very little research on cardiolipin and other phospholipids in lung injury, it is important to understand how cardiolipin and other phospholipids play a role in lung pathophysiology. In our study, we are the first to demonstrate that no significant difference in cell viability is shown in the treatment with phosphatidylcholine alone (300 uM) nor with a combination of cardiolipin (30uM) and phosphatidylcholine (70uM) in bovine pulmonary artery endothelial cells at 4 hr and 24 hr. However, cells treated with 30uM cardiolipin and 70 uM phosphatidylcholine for 24 hr have a significant increase in caspase-3 and caspase-7 activity. Furthermore, caspase-3 and caspase-7 activity was elevated by treatment with CL alone at 10uM and 30uM. Conclusions: Cardiolipin and co-treatment with phosphatidylcholine induces apoptosis pathways in caspase-3 and caspase-7 cascades in bovine pulmonary artery endothelial cells. Public health significance: This study provides insight into the potential role of cardiolipin in the pathophysiology of lung injury. This may further open new approaches for the development of therapeutic intervention for pulmonary diseases.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Liu, Shannen Yuan-Chunshannenliu@gmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairPitt, Bruce Rbrucep@pitt.eduBRUCEP
Committee MemberCroix, Claudette M. Stcls13@pitt.eduCLS13
Committee MemberWatkins, Simon Cswatkins@pitt.eduSWATKINS
Date: 27 September 2010
Date Type: Completion
Defense Date: 9 July 2010
Approval Date: 27 September 2010
Submission Date: 22 July 2010
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Environmental and Occupational Health
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: cytotoxicity; Bovine Pulmonary Artery Endothelial Cells; Cardiolipin; pulmonary endothelium
Other ID: http://etd.library.pitt.edu/ETD/available/etd-07222010-135953/, etd-07222010-135953
Date Deposited: 10 Nov 2011 19:52
Last Modified: 15 Nov 2016 13:46
URI: http://d-scholarship.pitt.edu/id/eprint/8522

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