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VB EXPRESSION ANALYSIS IN TYPE 1 DIABETES POPULATION AND THEIR UNAFFECTED FIRST DEGREE RELATIVES: A PRELIMINARY ANALYSIS

Han, Mei (2008) VB EXPRESSION ANALYSIS IN TYPE 1 DIABETES POPULATION AND THEIR UNAFFECTED FIRST DEGREE RELATIVES: A PRELIMINARY ANALYSIS. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Type 1 diabetes (T1D) is an autoimmune disease and is one of the most common diseases of children. It has a significant impact on public health costing millions of dollars in health care every year. Some studies have found that diabetes-associated T-cell receptor (TCR) bias can be detected in the peripheral circulation, and precede the onset of T1D by years. Therefore confirmation of this postulate would identify an acceleration time point that could be targeted by intervention strategies. The overall purpose of the study is to investigate VB expressions in T1D. This thesis is a preliminary analysis to explore the basic relationships among the various data points and provides direction for the investigators. Results show that VB1 and VB13.1 expressions for new onsets were correlated with the number of days since diagnosis. Significantly higher values were observed within 7 days of diagnosis compared to those measured further away. In comparison to FDRs, new onsets with blood drawn within 7 days of diagnosis showed higher VB1 values. Male FDRs had higher VB1 expression than female FDRs. The VB7 expression among black new onsets was significantly higher than for whites. Black FDRs showed higher VB1 expression and lower VB13.1 expression than white FDRs. Our data suggests that VB expression does not correlate with antibody status (GAD, IA2, IAA, and ICA) or the number of positive antibodies. Moreover, new onsets and FDRs show no significant differences in VB expression among individuals with different non-ASP status, DQ2 and DQ8 alleles. Logistic regression models that included all FDRs and new onsets with VB data available within 7 days of diagnosis suggest that VB1, VB13.1, DQ8 and age are associated with the onset of diabetes. Thus, VB expression is an independent predictor of T1D. We recommend that when designing studies to assess VB expression and analyzing the resultant data, it is important to consider the timing of the blood draws and the number of days since diagnosis. Our evidence suggests that VB expression is higher at onset and then decreases over time and needs to be taken into account in the design stage and the analysis.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Han, Meihanmei168@hotmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairArena, Vincent Carena@pitt.eduARENA
Committee MemberLibman, Ingrid MIngrid.Libman@chp.eduIML1
Committee MemberLuppi, Patrizialuppip@pitt.eduLUPPIP
Date: 28 September 2008
Date Type: Completion
Defense Date: 8 August 2008
Approval Date: 28 September 2008
Submission Date: 1 August 2008
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Biostatistics
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: the first degree relatives (FDRs); VB expression; new onsets; Type 1 diabetes
Other ID: http://etd.library.pitt.edu/ETD/available/etd-08012008-140420/, etd-08012008-140420
Date Deposited: 10 Nov 2011 19:56
Last Modified: 19 Dec 2016 14:36
URI: http://d-scholarship.pitt.edu/id/eprint/8801

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