Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

LTP- and LTD-inducing stimulations cause opposite changes in Arc/Arg3.1 mRNA level in hippocampal area CA1 in vivo

Yilmaz, Eser (2010) LTP- and LTD-inducing stimulations cause opposite changes in Arc/Arg3.1 mRNA level in hippocampal area CA1 in vivo. Master's Thesis, University of Pittsburgh. (Unpublished)

Primary Text

Download (685kB) | Preview


Immediate early genes (IEGs) typically are the first genetic responders to a variety of cellular activations. The IEG that encodes activity-regulated cytoskeleton-associated protein (arc/arg3.1) has attracted much interest because its mRNA is transported to and translated near activated synapses. Moreover, arc has been implicated in both long-term potentiation (LTP) and long-term depression (LTD). However, little is known about the time course of altered arc expression during LTP and LTD. Here we characterized arc mRNA levels in area CA1 of the adult rat hippocampus in vivo after LTP- and LTD-inducing stimulations that were identical except for the temporal patterning of the stimulation pulses. We observed a persistent increase in arc mRNA level during LTP. In contrast, during LTD, arc mRNA level first was decreased and then transiently increased relative to control level. These findings demonstrate that arc mRNA is regulated differently during LTP and LTD, and they provide evidence for stimulation-induced down-regulation of mRNA availability during LTD. Findings of abbreviated LTD when transcription was inhibited indicate that the prolonged maintenance of the NMDA receptor-dependent LTD studied here requires de novo transcription. Furthermore, findings of no LTD-associated change in the mRNA level of the IEG zif268 show that the decrease in arc mRNA during LTD is not a general genetic response. Thus, the regulation of arc expression not only differs between LTP and LTD but also diverges from that of other IEGs implicated in activity-dependent synaptic plasticity.


Social Networking:
Share |


Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Yilmaz, Eseresy4@pitt.eduESY4
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairDeFranco, Donald Bdod1@pitt.eduDOD1
Committee MemberThiels, Eddathiels@neurobio.pitt.eduTHIELS
Committee MemberBarrionuevo, Germangerman@pitt.eduGERMAN
Date: 31 August 2010
Date Type: Completion
Defense Date: 5 May 2010
Approval Date: 31 August 2010
Submission Date: 6 August 2010
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Neurobiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: immediate early gene; qPCR; synaptic plasticity; zif268
Other ID:, etd-08062010-083325
Date Deposited: 10 Nov 2011 19:57
Last Modified: 15 Nov 2016 13:48


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item