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Repression of Dpp targets in the Drosophila wing by Brinker

Winter, Stephanie E. (2005) Repression of Dpp targets in the Drosophila wing by Brinker. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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During development, gradients of transcription factors regulate patterning along a body axis by activation or repression of target genes above distinct threshold levels. In the Drosophila wing, expression of genes such as spalt (sal) and optomotor-blind (omb) is restricted along the A/P axis by lateral-to-medial gradients of the transcriptional repressor Brinker (Brk). sal is more sensitive to repression by Brk than omb, and thus has a narrower expression domain compared to omb. The Brk expression pattern is established by Decapentaplegic (Dpp), a TGF-b superfamily member, which forms a complementary medial-to-lateral morphogen gradient along the (A/P) axis of the wing. We have investigated the mechanisms Brk uses to repress gene expression to gain an understanding of why some targets such as sal are more sensitive to Brk than others such as omb. Previous studies have suggested that Brk represses gene expression simply by competing with activators, but we show that Brk requires an active repression domain along with the DNA binding domain for repressor activity. Brk possesses four repression domains, but these domains are not equivalent; for example, 3R is sufficient to repress omb but not sal. Consequently, although sal and omb show quantitative differences in their response to Brk, there are qualitative differences in the mechanisms that Brk uses to repress each of these genes.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Winter, Stephanie E.swinter@pitt.eduSWINTER
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairCampbell, Gerard Lcamp@pitt.eduCAMP
Committee MemberLopez, A.
Committee MemberStronach, Bethstronach@pitt.eduSTRONACH
Committee MemberBrodsky, Jeffreyjbrodsky@pitt.eduJBRODSKY
Committee MemberGrabowski, Paula Jpag4@pitt.eduPAG4
Date: 10 October 2005
Date Type: Completion
Defense Date: 25 July 2005
Approval Date: 10 October 2005
Submission Date: 15 August 2005
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Brinker; Dpp; Drosophila; transcriptional repression
Other ID:, etd-08152005-134633
Date Deposited: 10 Nov 2011 19:59
Last Modified: 15 Nov 2016 13:49


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