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Lipoprotein lipase gene sequencing and plasma lipid profile

Pirim, Dilek (2011) Lipoprotein lipase gene sequencing and plasma lipid profile. Master's Thesis, University of Pittsburgh.

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    Abstract

    In the United States, coronary heart disease (CHD) is the most common cause of death and number one killer of American males and females. Several epidemiological studies have identified risk factors for CHD, like low high-density lipoprotein cholesterol (HDL-C), elevated total cholesterol and low-density lipoprotein (LDL) cholesterol, and high triglycerides (TGs), but underlying genetic variations that cause predisposition to these traits still remain unclear. Lipoprotein lipase (LPL) is one of the major genes involved in lipid metabolism and its gene sequence variation has already been reported to be associated with the risk of CHD and risk of other complex diseases like dyslipidemia, type 2 diabetes, essential hypertension, and Alzheimer's disease. Unraveling the unknown genetic variation in the LPL gene in relation to HDL-C and correlated lipid traits is critically important for public health because identification of genetic markers may lead to promising future public health interventions, like prognostic tools and therapeutic approaches to alleviate the burden of CHD in the U.S. In this study, we investigated the role of common and rare variation in LPL by resequencing individuals having extremely low (n=48) and high (n=47) HDL-C levels selected from a population-based non-Hispanic white (NHW) sample of 623 individuals. A total of 179 variants were identified in 95 individuals by resequencing the entire LPL gene, including 91 uncommon or rare variants [minor allele frequency (MAF) <0.05)] and 88 common variants (MAF &#8805; 0.05). Of the 91 relatively uncommon or rare variants, 21 were present only in the low-HDL group and 25 were present only in the high HDL-C group. Overall, the prevalence of uncommon or rare variants was higher in the high HDL-C than the low HDL-C group. Thirty two of the 88 common variants demonstrated significant association (P-value <0.05) between the high and low HDL-C groups. We also examined 12 common variants (MAF &#8805; 0.05) in the total NHW sample and identified 7 variants to be significantly associated with lipid levels. In conclusion, our comprehensive resequencing of the LPL gene confirms that both common and rare variants in this gene are associated with interindividual variation in plasma lipid profile.


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    Item Type: University of Pittsburgh ETD
    ETD Committee:
    ETD Committee TypeCommittee MemberEmail
    Committee ChairKamboh, M. Ilyaskamboh@pitt.edu
    Committee MemberBunker, Clareann HBUNKERC@pitt.edu
    Committee MemberDemirci, F. Yesimfyd1@pitt.edu
    Title: Lipoprotein lipase gene sequencing and plasma lipid profile
    Status: Unpublished
    Abstract: In the United States, coronary heart disease (CHD) is the most common cause of death and number one killer of American males and females. Several epidemiological studies have identified risk factors for CHD, like low high-density lipoprotein cholesterol (HDL-C), elevated total cholesterol and low-density lipoprotein (LDL) cholesterol, and high triglycerides (TGs), but underlying genetic variations that cause predisposition to these traits still remain unclear. Lipoprotein lipase (LPL) is one of the major genes involved in lipid metabolism and its gene sequence variation has already been reported to be associated with the risk of CHD and risk of other complex diseases like dyslipidemia, type 2 diabetes, essential hypertension, and Alzheimer's disease. Unraveling the unknown genetic variation in the LPL gene in relation to HDL-C and correlated lipid traits is critically important for public health because identification of genetic markers may lead to promising future public health interventions, like prognostic tools and therapeutic approaches to alleviate the burden of CHD in the U.S. In this study, we investigated the role of common and rare variation in LPL by resequencing individuals having extremely low (n=48) and high (n=47) HDL-C levels selected from a population-based non-Hispanic white (NHW) sample of 623 individuals. A total of 179 variants were identified in 95 individuals by resequencing the entire LPL gene, including 91 uncommon or rare variants [minor allele frequency (MAF) <0.05)] and 88 common variants (MAF &#8805; 0.05). Of the 91 relatively uncommon or rare variants, 21 were present only in the low-HDL group and 25 were present only in the high HDL-C group. Overall, the prevalence of uncommon or rare variants was higher in the high HDL-C than the low HDL-C group. Thirty two of the 88 common variants demonstrated significant association (P-value <0.05) between the high and low HDL-C groups. We also examined 12 common variants (MAF &#8805; 0.05) in the total NHW sample and identified 7 variants to be significantly associated with lipid levels. In conclusion, our comprehensive resequencing of the LPL gene confirms that both common and rare variants in this gene are associated with interindividual variation in plasma lipid profile.
    Date: 31 January 2011
    Date Type: Completion
    Defense Date: 08 December 2010
    Approval Date: 31 January 2011
    Submission Date: 30 November 2010
    Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
    Patent pending: No
    Institution: University of Pittsburgh
    Thesis Type: Master's Thesis
    Refereed: Yes
    Degree: MS - Master of Science
    URN: etd-11302010-184759
    Uncontrolled Keywords: coronary heart disease; HDL-C; lipid metabolism; lipoprotein lipase; LPL; Triglycerides; HDL metabolism; lipid levels
    Schools and Programs: Graduate School of Public Health > Human Genetics
    Date Deposited: 10 Nov 2011 15:07
    Last Modified: 15 May 2012 14:24
    Other ID: http://etd.library.pitt.edu/ETD/available/etd-11302010-184759/, etd-11302010-184759

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