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Impact of limited solvent capacity on metabolic rate, enzyme activities, and metabolite concentrations of S. cerevisiae glycolysis

Vazquez, A and De Menezes, MA and Barabási, AL and Oltvai, ZN (2008) Impact of limited solvent capacity on metabolic rate, enzyme activities, and metabolite concentrations of S. cerevisiae glycolysis. PLoS Computational Biology, 4 (10). ISSN 1553-734X

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Abstract

The cell's cytoplasm is crowded by its various molecular components, resulting in a limited solvent capacity for the allocation of new proteins, thus constraining various cellular processes such as metabolism. Here we study the impact of the limited solvent capacity constraint on the metabolic rate, enzyme activities, and metabolite concentrations using a computational model of Saccharomyces cerevisiae glycolysis as a case study. We show that given the limited solvent capacity constraint, the optimal enzyme activities and the metabolite concentrations necessary to achieve a maximum rate of glycolysis are in agreement with their experimentally measured values. Furthermore, the predicted maximum glycolytic rate determined by the solvent capacity constraint is close to that measured in vivo. These results indicate that the limited solvent capacity is a relevant constraint acting on S. cerevisiae at physiological growth conditions, and that a full kinetic model together with the limited solvent capacity constraint can be used to predict both metabolite concentrations and enzyme activities in vivo. © 2008 Vazquez et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Vazquez, A
De Menezes, MA
Barabási, AL
Oltvai, ZNoltvai@pitt.eduOLTVAI
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorPapin, Jason A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 October 2008
Date Type: Publication
Journal or Publication Title: PLoS Computational Biology
Volume: 4
Number: 10
DOI or Unique Handle: 10.1371/journal.pcbi.1000195
Refereed: Yes
ISSN: 1553-734X
PubMed Central ID: PMC2533405
PubMed ID: 18846199
Date Deposited: 24 Jul 2012 18:44
Last Modified: 02 Feb 2019 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/13001

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