Bivona, Kevin
(2012)
DIFFUSION IMAGING OF LIPID MEMBRANES: DEVELOPING MULTI-PULSE NMR TO IMAGE STATIC MEMBRANES SAMPLES.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
A multi-pulse (MP), pulsed-field gradient (PG) stimulated echo (STE) nuclear magnetic resonance (NMR) pulse program was constructed and refined using ethylene glycol (EG), with
the goal of providing a detailed diffusion image of a lipid membrane. This MP PGSTE NMR technique offers a useful combination of magnetic resonance imaging (MRI) and NMR tools that may provide images of lipid rafts at a length scale that is not restricted by the diffraction limit. MP NMR was used to provide homonuclear dipole-dipole decoupling, directly replacing magic-angle spinning (MAS), in a PGSTE NMR pulse program. Optimizing the use of MP NMR for EG and lipids, it was found that moving the carrier frequency ~2.5 kHz off resonance and shortening the cycle time to the minimum allowed by the probe hardware (in this case 43.2 μs at a power level of 139 kHz) produced the best results. By eliminating the use of magic-angle spinning (MAS), the gradient coils in the NMR diffusion-imaging probe may be placed closer to the sample, increasing their effect. Future work is needed to apply MP PGSTE NMR to static lipid samples. A basic PGSTE NMR diffusion measuring pulse program was used to determine the self-diffusion coefficient, D, of EG with the results showing Dself for EG was 6.5 x 10^-11 m^2 s^-1.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
19 September 2012 |
| Date Type: |
Publication |
| Defense Date: |
30 March 2012 |
| Approval Date: |
19 September 2012 |
| Submission Date: |
19 July 2012 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
67 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
solid state NMR nuclear magnetic resonance multi-pulse diffusion imaging lipid membranes rafts |
| Date Deposited: |
19 Sep 2012 18:01 |
| Last Modified: |
15 Nov 2016 14:00 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/13014 |
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