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Whole genome sequencing to investigate the emergence of clonal complex 23 Neisseria meningitidis serogroup Y disease in the United States

Krauland, MG and Hotopp, JCD and Riley, DR and Daugherty, SC and Marsh, JW and Messonnier, NE and Mayer, LW and Tettelin, H and Harrison, LH (2012) Whole genome sequencing to investigate the emergence of clonal complex 23 Neisseria meningitidis serogroup Y disease in the United States. PLoS ONE, 7 (4).

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Abstract

In the United States, serogroup Y, ST-23 clonal complex Neisseria meningitidis was responsible for an increase in meningococcal disease incidence during the 1990s. This increase was accompanied by antigenic shift of three outer membrane proteins, with a decrease in the population that predominated in the early 1990s as a different population emerged later in that decade. To understand factors that may have been responsible for the emergence of serogroup Y disease, we used whole genome pyrosequencing to investigate genetic differences between isolates from early and late N. meningitidis populations, obtained from meningococcal disease cases in Maryland in the 1990s. The genomes of isolates from the early and late populations were highly similar, with 1231 of 1776 shared genes exhibiting 100% amino acid identity and an average πN = 0.0033 and average πS = 0.0216. However, differences were found in predicted proteins that affect pilin structure and antigen profile and in predicted proteins involved in iron acquisition and uptake. The observed changes are consistent with acquisition of new alleles through horizontal gene transfer. Changes in antigen profile due to the genetic differences found in this study likely allowed the late population to emerge due to escape from population immunity. These findings may predict which antigenic factors are important in the cyclic epidemiology of meningococcal disease.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Krauland, MGmgk8@pitt.eduMGK8
Hotopp, JCD
Riley, DR
Daugherty, SC
Marsh, JWjwmarsh@pitt.eduJWMARSH
Messonnier, NE
Mayer, LW
Tettelin, H
Harrison, LHlharriso@edc.pitt.eduLHARRISO
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorBorrow, RayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 27 April 2012
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 7
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0035699
Schools and Programs: School of Public Health > Infectious Diseases and Microbiology
School of Medicine > Infectious Diseases and Microbiology
Refereed: Yes
Other ID: NLM PMC3338715
PubMed Central ID: PMC3338715
PubMed ID: 22558202
Date Deposited: 24 Sep 2012 20:21
Last Modified: 22 Jun 2021 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/14178

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