The role of iron in Mycobacterium smegmatis biofilm formation: The exochelin siderophore is essential in limiting iron conditions for biofilm formation but not for planktonic growth

Ojha, A and Hatfull, GF (2007) The role of iron in Mycobacterium smegmatis biofilm formation: The exochelin siderophore is essential in limiting iron conditions for biofilm formation but not for planktonic growth. Molecular Microbiology, 66 (2). 468 - 483. ISSN 0950-382X

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Abstract

Many species of mycobacteria form structured biofilm communities at liquid-air interfaces and on solid surfaces. Full development of Mycobacterium smegmatis biofilms requires addition of supplemental iron above 1 μM ferrous sulphate, although addition of iron is not needed for planktonic growth. Microarray analysis of the M. smegmatis transcriptome shows that iron-responsive genes - especially those involved in siderophore synthesis and iron uptake - are strongly induced during biofilm formation reflecting a response to iron deprivation, even when 2 μM iron is present. The acquisition of iron under these conditions is specifically dependent on the exochelin synthesis and uptake pathways, and the strong defect of an iron-exochelin uptake mutant suggests a regulatory role of iron in the transition to biofilm growth. In contrast, although the expression of mycobactin and iron ABC transport operons is highly upregulated during biofilm formation, mutants in these systems form normal biofilms in low-iron (2 μM) conditions. A close correlation between iron availability and matrix-associated fatty acids implies a possible metabolic role in the late stages of biofilm maturation, in addition to the early regulatory role. M. smegmatis surface motility is similarly dependent on iron availability, requiring both supplemental iron and the exochelin pathway to acquire it. © 2007 The Authors.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Ojha, A ano7@pitt.edu ANO7 Hatfull, GF gfh@pitt.edu GFH
Date:	1 October 2007
Date Type:	Publication
Journal or Publication Title:	Molecular Microbiology
Volume:	66
Number:	2
Page Range:	468 - 483
DOI or Unique Handle:	10.1111/j.1365-2958.2007.05935.x
Schools and Programs:	Dietrich School of Arts and Sciences > Biological Sciences
Refereed:	Yes
ISSN:	0950-382X
MeSH Headings:	Base Sequence; Biofilms--growth & development; Fatty Acids--metabolism; Gene Expression Regulation, Bacterial--drug effects; Iron--pharmacology; Models, Genetic; Molecular Sequence Data; Mycobacterium smegmatis--drug effects; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--growth & development; Oligonucleotide Array Sequence Analysis; Peptides, Cyclic--genetics; Peptides, Cyclic--physiology; Plankton--drug effects; Plankton--growth & development; Reverse Transcriptase Polymerase Chain Reaction; Siderophores--genetics; Siderophores--physiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Other ID:	NLM PMC2170428
PubMed Central ID:	PMC2170428
PubMed ID:	17854402
Date Deposited:	03 Dec 2012 22:38
Last Modified:	02 Feb 2019 16:56
URI:	http://d-scholarship.pitt.edu/id/eprint/16383

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