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Enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms.

Ojha, Anil K and Trivelli, Xavier and Guerardel, Yann and Kremer, Laurent and Hatfull, Graham F (2010) Enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms. J Biol Chem, 285 (23). 17380 - 17389.

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Abstract

Mycobacterial species, like other microbes, spontaneously form multicellular drug-tolerant biofilms when grown in vitro in detergent-free liquid media. The structure of Mycobacterium tuberculosis biofilms is formed through genetically programmed pathways and is built upon a large abundance of novel extracellular free mycolic acids (FM), although the mechanism of FM synthesis remained unclear. Here we show that the FM in Mycobacterium smegmatis biofilms is produced through the enzymatic release from constitutively present mycolyl derivatives. One of the precursors for FM is newly synthesized trehalose dimycolate (TDM), which is cleaved by a novel TDM-specific serine esterase, Msmeg_1529. Disruption of Msmeg_1529 leads to undetectable hydrolytic activity, reduced levels of FM in the mutant, and retarded biofilm growth. Furthermore, enzymatic hydrolysis of TDM remains conserved in M. tuberculosis, suggesting the presence of a TDM-specific esterase in this pathogen. Overall, this study provides the first evidence for an enzymatic release of free mycolic acids from cell envelope mycolates during mycobacterial growth.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ojha, Anil Kano7@pitt.eduANO7
Trivelli, Xavier
Guerardel, Yann
Kremer, Laurent
Hatfull, Graham Fgfh@pitt.eduGFH
Date: 4 June 2010
Date Type: Publication
Journal or Publication Title: J Biol Chem
Volume: 285
Number: 23
Page Range: 17380 - 17389
DOI or Unique Handle: 10.1074/jbc.m110.112813
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Uncontrolled Keywords: Amino Acid Sequence, Biofilms, Cloning, Molecular, Cord Factors, Esterases, Hydrolysis, Lipids, Models, Genetic, Molecular Sequence Data, Mutation, Mycobacterium, Mycobacterium tuberculosis, Mycolic Acids, Phenotype, Sequence Homology, Amino Acid
Funders: NIAID NIH HHS (R01 AI064494), NIAID NIH HHS (R21 AI079288), NIAID NIH HHS (AI064494), NIAID NIH HHS (AI079288)
MeSH Headings: Amino Acid Sequence; Biofilms; Cloning, Molecular; Cord Factors--chemistry; Esterases--chemistry; Hydrolysis; Lipids--chemistry; Models, Genetic; Molecular Sequence Data; Mutation; Mycobacterium--metabolism; Mycobacterium tuberculosis--metabolism; Mycolic Acids--chemistry; Phenotype; Sequence Homology, Amino Acid
Other ID: NLM PMC2878501
PubMed Central ID: PMC2878501
PubMed ID: 20375425
Date Deposited: 19 Nov 2012 16:47
Last Modified: 27 Sep 2022 15:02
URI: http://d-scholarship.pitt.edu/id/eprint/16393

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